Clonal evolution in patients with gain/amplification of 1q21. (A) The change in cell fraction and copy number of 1q21 gain between the 2 time-point samplings. Different color demonstrates 6 different changing patterns. (B) Failure-free survival from second sampling among patients with different 1q21 evolutionary patterns. (C) Six 1q21 evolutionary patterns are merged to 3 groups according to the survival curves. Kaplan-Meier plots for first FFS, second FFS, first OS, and second OS are presented in patients with different groups. m, months. (D) Diagram of different kinds of evolution in copy number or clone size of 1q21 gain during follow-up.