Top 20 CD8⁺ T-cell clones are expanding in patients with GVL. (A) CD8⁺TRB repertoire proportions are compared between patients with GVL effect (green; n = 14) and those without GVL (orange; n = 11). Two time points are compared, with closed circles displaying the pre-GVL/noGVL time point and closed squares displaying the time point of first onset of GVL/noGVL. (B) Difference in CD8⁺TRB repertoire (y-axis) occupied by top 20, 21 to 50, and 51 to N clones between pre-GVL/noGVL and first GVL/noGVL time. (C-D) Clonal proportions of the CD8⁺TRB repertoire (y-axis) are shown over time for 3 consecutive time points (x-axis). Every single CDR3 sequence of the top 20 clones is shown in a different color, as indicated by the legend. The top 21 to 50 (gray) and 51 to N clones (hatched area) are grouped together. The arrow indicates the time point of administration of DLI. Displayed are 2 representative patients without GVL (C) and 2 with GVL effect (D). Statistical analysis was performed by Wilcoxon matched-pairs signed ranked test (two-tailed) (A) and Mann-Whitney test (two-tailed) (B). Black lines represent median; error bars show the interquartile range. *P < .05; **P < .01; ns, not significant.