LSD1 LOF shifts erythroid differentiation potential to the GM lineage. Representative flow cytometry plots showing the gating strategies for LSK cells, CMPs, GMPs, and MEPs (A) and their absolute cell numbers (B) in Tx-treated Lsd1 CKO or control mouse BM cells. (C) TdTomato epifluorescence was analyzed in individual cell populations of Tx-treated R26T:G1BCreER^T2 control mice (blue graphs) or Lsd1 CKOT mice (red graphs). (D) Erythroid progenitors give rise to GM colonies in LSD1-CKO BM. Sorted CFU-E, pre–CFU-E, and pre-MegE cells from Tx-treated control and Lsd1 CKO mice were pooled for seeding in colony-forming unit assays. (E) Colony types (Meg, mixed MegE, GM, and BFU-E per 600 sorted cells) were quantified. (F) Representative flow cytometry plots of pooled GM colonies picked from Lsd1-CKO mice. The cells were stained with anti-Gr1, anti-CD11b, anti-Ter119, or anti-CD71 antibody. (G) Colony numbers of Megs, MegEs, CFU-GM, and BFU-E (per 600 sorted cells) from control mice treated with DMSO or different concentrations of CCG50 LSD1i. Data are mean ± standard deviation from 3 mice. **P < .01, ***P < .001, unpaired Student t test.