Specific GEP patterns for PB-DLBCL and NO-DLBCL-GCB. (A) A penalized logistic regression model assigned 34 differentially expressed genes between PB-DLBCL and NO-DLBCL-GCB. As shown in the heatmap, unsupervised hierarchical clustering of these differentially expressed genes generated 3 clusters: a cluster with predominant PB-DLBCLs, a cluster with an agglomeration of O-DLBCL subentities and NO-DLBCL-GCB, and a cluster with mainly NO-DLBCL-GCBs. In contrast to NO-DLBCL-GCB, PB-DLBCL exhibited significantly (P < .001) increased expression of immune response genes (CTLA4, CXCL12, HLA-A, HLA-C, HLA-E, and HLA-F). Elevated expressions of ARID1A and SMARCA4 (both involved in chromosome organization) and FOXO1 (a centroblast hallmark) were found in NO-DLBCL-GCBs, as opposed to low expressions in PB-DLBCLs. (B) This bar chart shows the distribution of the PB-DLBCL and NO-DLBCL-GCB subentities across elucidated clusters.