Thrombin generation analysis. Calibrated automated thrombography was performed using varying concentrations of recombinant hFIX (blue squares and lines) or An96-Padua (red circles and lines) spiked into hemophilia B plasma spanning the severe hemophilia B boundary (∼1%) to 100% normal pooled plasma hFIX range (0.05-5 μg/mL). Concentration-response curves of endogenous thrombin potential (ETP) (C), peak thrombin level (D), and lag time (E) for recombinant An96-Padua and hFIX are depicted.