Arhgef2^−/− bone marrow HSPCs are not altered in their total colony output, proliferation, cell cycle, or apoptosis status. (A) Myeloid CFUs from 1.2 × 10⁴ WBM cells plated in biological triplicate enumerated at 7 to 10 days. (B) LSK HSPCs cultured in vitro enumerated for proliferation (left), proportional G₁ (middle), and S/G₂/M (right) cell cycle status. (C) Early apoptosis (left) and late apoptosis at days 4 and 7 in culture (right). (B-C) Total of n = 6 biological replicates. Error bars represent SEM. *P < .05. BFU-E, burst forming unit-erythroid; CFU-GM, CFU-granulocyte–macrophage; CFU-M, CFU-megakaryocyte.