Gata2b-deficient HSPC1s show coexpression of myeloid and lymphoid gene expression programs. (A) Bar graph representing the percentage of WT HSPC1s in white and gata2b^−/− HSPC1s in gray, with at least 1 read of s100a10b, grn1, colony stimulating factor 3b (csf3bs), CCAAT enhancer binding protein α (cebpa), lysozyme (lyz), or actin beta1 (actb1), indicating more cells with detectable myeloid gene expression in Gata2b-deficient HSPC1s. Coexpression analysis of the lymphoid gene igic1s1 with the myeloid gene cebpb (B) and the lymphoid gene ikzf1 (C) with the myeloid gene s100a10b. Values represent percentages of WT and gata2b^−/− HSPC1s coexpressing myeloid and lymphoid genes (within the dashed box). WT and gata2b^−/− feature analysis representing coexpression analysis of the lymphoid gene ighv1-4, with the myeloid gene grn1 (D) and the phagocytic B-cell marker immunoglobulin light ι constant 1-s1 (igic1s1) with the myeloid gene s100a10b (E), with myeloid genes in blue and lymphoid genes in green. Coexpression of the myeloid and lymphoid genes is represented in turquoise and indicated by red arrows in the WT and gata2b^−/− feature analysis. Coloring threshold set in quantiles, with minimum cutoff of q10 and maximum cutoff of q90.