Enhanced hemostatic effect of FVIII-QQ relative to FVIII-WT is APC dependent. HA/FVL mice were infused with PBS (open diamonds), FVIII-WT (blue squares), or FVIII-QQ (red triangles) at 2 µg/kg with or without 10 mg/kg of mAPC anticoagulatant inhibitory antibody (mAb 1609) as indicated and then underwent tail clip injury. Each point represents 1 mouse, and medians with interquartile ranges are presented. Kruskal-Wallis test was used to determine significance relative to the FVL PBS controls, with P values ≤.1 considered significant. *P ≤ .1, **P ≤ .05, ***P ≤ .01. n.s., not significant.