Figure 6.
MYC-N11S polymorphism leads to disruption of the Y69 binding and reduced IHC staining. The N11S variant was modeled in DOHH-2 cells using CRISPR/Cas9. N11S−/− refers to WT cell lines, whereas N11S+/− and N11S+/+ refer to cell lines heterozygous and homozygous for the N11S variant, respectively. (A) Western blotting for MYC was performed on whole-cell extracts of N11S and WT DOHH-2 cells using the Y69 and 9E10 antibodies. Histone H3 was used as a loading control. (B) Ratio of protein detected by western blotting using the Y69 and 9E10 antibodies, normalized for loading. Error bars indicate standard error. (C) MYC IHC using the Y69 antibody on FFPE section from N11S and WT DOHH-2 cell lines (40× objective lens). The percentage of positively stained cells for each whole section is shown in the upper right corner of the images.

MYC-N11S polymorphism leads to disruption of the Y69 binding and reduced IHC staining. The N11S variant was modeled in DOHH-2 cells using CRISPR/Cas9. N11S−/− refers to WT cell lines, whereas N11S+/− and N11S+/+ refer to cell lines heterozygous and homozygous for the N11S variant, respectively. (A) Western blotting for MYC was performed on whole-cell extracts of N11S and WT DOHH-2 cells using the Y69 and 9E10 antibodies. Histone H3 was used as a loading control. (B) Ratio of protein detected by western blotting using the Y69 and 9E10 antibodies, normalized for loading. Error bars indicate standard error. (C) MYC IHC using the Y69 antibody on FFPE section from N11S and WT DOHH-2 cell lines (40× objective lens). The percentage of positively stained cells for each whole section is shown in the upper right corner of the images.

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