![Sdy and Tmem163-KO display significant impairment of platelet aggregation and prolonged bleeding time. (A-F) Washed platelets from sdy+/−, sdy, B6 (WT, C57BL/6J), or Tmem163-KO mice were stimulated with various concentrations of collagen (sdy+/− vs sdy, 39.38% vs 11.04% [1.2 μg/mL], and 71.05% vs 9.71% [2.4 μg/mL], n = 4, 4, 3, and 3, respectively; B6 vs Tmem163-KO, 51.50% vs 13.80% [1.2 μg/mL], and 80.42% vs 33.65% [2.4 μg/mL], n = 4, 4, 4, and 4, respectively) (A-B,E) or thrombin (sdy+/− vs sdy, 31.91% vs 6.53% [0.025 U/mL], and 68.03% vs 27.78% [0.05 U/mL], n = 4, 5, 3, and 3, respectively; B6 vs Tmem163-KO, 21.60% vs 0.99% [0.025 U/mL], 65.01% vs 25.44% [0.05 U/mL], n = 9, 7, 7, and 6, respectively) (C-D,F) and aggregation assessed by a turbidimetric aggregometer. (G) Tail-bleeding assays showed Tmem163-KO mice have prolonged bleeding times compared with B6 mice (B6, 135 ± 27.59 s; n = 9; Tmem163-KO, >900 s; n = 12). Student t test, *P < .05; **P < .01; ***P < .001. OD, optic density.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/137/13/10.1182_blood.2020007389/1/bloodbld2020007389f6.png?Expires=1753580880&Signature=A8mxlp9GSFNw3Lqsep5CypbNgbjZ64m~LNNypnf3MccC~SQrYdOejH-LdZwALqdF2SZLSBXUmwUkl-zgt73-~1u4Emd6KrhHDULR0dURggu1wUkJg5n2XNyGZ9DuMW3lt6mQp4Lva6jOigXMkjG93qYz9SgU5i0sgisy7ClgSIFlQHvnVw87APmop6muEYaixu7xOAKIbW3mbxF2TCFK44Is5wlUr0FIb~l7QumsR2uZ6ApK38lXCN9gWkBvsx5XsqfNKMbX5wkbrOt~yZuMFZdJHDUd-bsBTeD23MlWzca4x0rFRje52QFWIT~51~csqSlvfomK48AMpgw7hCb4CQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Sdy and Tmem163-KO display significant impairment of platelet aggregation and prolonged bleeding time. (A-F) Washed platelets from sdy+/−, sdy, B6 (WT, C57BL/6J), or Tmem163-KO mice were stimulated with various concentrations of collagen (sdy+/− vs sdy, 39.38% vs 11.04% [1.2 μg/mL], and 71.05% vs 9.71% [2.4 μg/mL], n = 4, 4, 3, and 3, respectively; B6 vs Tmem163-KO, 51.50% vs 13.80% [1.2 μg/mL], and 80.42% vs 33.65% [2.4 μg/mL], n = 4, 4, 4, and 4, respectively) (A-B,E) or thrombin (sdy+/− vs sdy, 31.91% vs 6.53% [0.025 U/mL], and 68.03% vs 27.78% [0.05 U/mL], n = 4, 5, 3, and 3, respectively; B6 vs Tmem163-KO, 21.60% vs 0.99% [0.025 U/mL], 65.01% vs 25.44% [0.05 U/mL], n = 9, 7, 7, and 6, respectively) (C-D,F) and aggregation assessed by a turbidimetric aggregometer. (G) Tail-bleeding assays showed Tmem163-KO mice have prolonged bleeding times compared with B6 mice (B6, 135 ± 27.59 s; n = 9; Tmem163-KO, >900 s; n = 12). Student t test, *P < .05; **P < .01; ***P < .001. OD, optic density.