Table 3. Serologically predicted... | American Society of Hematology

Table 3.

Serologically predicted genotype, ethnicity of donors, dPCR RHD zygosity results, and RHD allele as determined by NGS for samples sequenced (n = 69)

Sample no.	Rh serology*	Ethnicity*	RHD5-to-AGO1 ratio	RHD7-to-AGO1 ratio	dPCR RHD zygosity	Allele
004_01	R₁R₁	Caucasian	1.12	1.05	Homozygous	RHD*01
004_02	R₁R₁	Caucasian	1.12	1.03	Homozygous	RHD*01
004_03	R₁R₁	Other	1.01	1.04	Homozygous	RHD*01
004_04	R₁R₁	Caucasian	1.07	1.03	Homozygous	RHD*01
004_05	R₁R₁	Caucasian	1.01	1.06	Homozygous	RHD*01
004_06	R₁R₁	Caucasian	0.99	1.04	Homozygous	RHD*01
004_07	R₁R₁	Caucasian	0.54	1.01	Discrepancy†	*RHD***01W.01*
004_08	R₁r	Caucasian	0.54	0.57	Hemizygous	RHD*01
004_09	R₁r	Caucasian	0.54	0.53	Hemizygous	RHD*01
004_10	R₁r	Chinese	0.51	0.56	Hemizygous	RHD*01
004_11	R₁r	Caucasian	0.53	0.54	Hemizygous	RHD*01
004_12	R₁r	Caucasian	0.55	0.52	Hemizygous	RHD*01
004_13	R₁r	Caucasian	0.54	0.6	Hemizygous	RHD*01
004_14	R₁r	Caucasian	1.06	0.99	Homozygous‡	*RHD**01
004_15	R₁r	Caucasian	0.53	0.50	Hemizygous	RHD*01W.01
004_16	R₁r	Caucasian	0.54	0.52	Hemizygous	RHD*01W.01
004_17	R₁r	Caucasian	0.58	0.56	Hemizygous	RHD*01W.01
004_18	R₁r	Caucasian	0.54	0.52	Hemizygous	RHD*01W.01
004_19	R₁r	Caucasian	0.54	0.47	Hemizygous	RHD*01W.01
004_20	R₁r	Caucasian	0.53	0.57	Hemizygous	RHD*01W.01
004_21	R₁r	Caucasian	0.52	0.57	Hemizygous	RHD*01W.01
004_22	R₁r	Caucasian	0.54	0.53	Hemizygous	RHD*01W.01
004_23	R₁r	Caucasian	0.52	0.53	Hemizygous	RHD*01W.01
004_24	R₁r	Caucasian	0.53	0.54	Hemizygous	RHD*01W.01
004_25	R₁r	Caucasian	0.53	0.51	Hemizygous	RHD*01W.01
004_26	R₁r	Caucasian	0.57	0.52	Hemizygous	RHD*01W.01
004_27	R₁r	Caucasian	0.56	0.54	Hemizygous	RHD*01W.01
004_28	R₁r	Caucasian	0.53	0.52	Hemizygous	RHD*01W.03
004_29	R₁R₂	Caucasian	1.09	1.03	Homozygous	RHD*01
004_30	R₁R₂	Caucasian	0.95	0.94	Homozygous	RHD*01
004_31	R₁R₂	Caucasian	1.08	1.05	Homozygous	RHD*01
004_32	R₁R₂	Caucasian	0.97	1.04	Homozygous	RHD*01
004_33	R₁R₂	Caucasian	1.03	1.08	Homozygous	RHD*01
004_34	R₁R₂	Caucasian	0.98	1.08	Homozygous	RHD*01
004_35	R₁R₂	Caucasian	0.46	0.51	Hemizygous§	*RHD***01W.02*
004_36	R₁R₂	Caucasian	0.51	0.51	Hemizygous§	*RHD**01
004_37	R₁R₂	Caucasian	0.53	0.49	Hemizygous§	*RHD**01
004_38	R₁R₂	Caucasian	0.51	0.53	Hemizygous§	*RHD**01
004_39	R₁R₂	Caucasian	0.52	0.51	Hemizygous§	*RHD**01
004_40	R₁R₂	Caucasian	0.53	0.52	Hemizygous§	*RHD**01
004_41	R₂R₂	Caucasian	1.01	0.99	Homozygous	RHD*01W.02
004_42	R₂R₂	Not disclosed	0.54	0.47	Hemizygous\|\|	*RHD***01W.02*
004_43	R₂R₂	Caucasian	1.01	1.02	Homozygous	RHD*01
004_44	R₂R₂	Caucasian	1.01	0.99	Homozygous	RHD*01
004_45	R₂R₂	Caucasian	1.03	1.02	Homozygous	RHD*01
004_46	R₂R₂	Caucasian	1.02	1.01	Homozygous	RHD*01
004_47	R₂R₂	Caucasian	1.01	1	Homozygous	RHD*01
004_48	R₂r	Caucasian	0.53	0.54	Hemizygous	RHD*01
004_49	R₂r	Caucasian	0.53	0.51	Hemizygous	RHD*01
004_50	R₂r	Caucasian	0.53	0.51	Hemizygous	RHD*01
004_51	R₂r	Caucasian	0.52	0.56	Hemizygous	RHD*01
004_52	R₂r	Caucasian	0.48	0.54	Hemizygous	RHD*01
004_53	R₂r	Caucasian	0.53	0.47	Hemizygous	RHD*01
004_54	R₂r	Caucasian	0.52	0.51	Hemizygous	RHD*01W.02
004_55	R₂r	Caucasian	0.53	0.52	Hemizygous	RHD*01W.02
004_56	R₂r	Caucasian	0.53	0.5	Hemizygous	RHD*01W.02
004_57	R₂r	Caucasian	0.51	0.57	Hemizygous	RHD*01W.02
004_58	R₂r	Caucasian	0.58	0.56	Hemizygous	RHD*01W.02
004_59	R₂r	Caucasian	0.51	0.53	Hemizygous	RHD*01W.02
004_60	R₂r	Caucasian	0.57	0.52	Hemizygous	RHD*01W.02
004_61	R₂r	Caucasian	0.55	0.53	Hemizygous	RHD*01W.02
004_62	R₂r	Caucasian	0.58	0.52	Hemizygous	RHD*01W.02
004_63	R₀r	Caucasian	0.46	0.46	Hemizygous	RHD*01
004_64	R₀r	Caucasian	0.5	0.53	Hemizygous	RHD*01
004_65	R₀r	Caucasian	0.46	0.49	Hemizygous	RHD*01
004_66	R₀r	Caucasian	0.49	0.52	Hemizygous	RHD*01
004_67	R₀r	Caucasian	0.53	0.51	Hemizygous	RHD*01
004_68	R₀r	Caucasian	0.52	0.51	Hemizygous	RHD*01
004_69	R₂R_Z	Caucasian	1.02	1.02	Homozygous	RHD*01
004_70- 004_123	R₁R₂	—	1.0¶	1.0¶	Homozygous	Not sequenced

Sample no.	Rh serology*	Ethnicity*	RHD5-to-AGO1 ratio	RHD7-to-AGO1 ratio	dPCR RHD zygosity	Allele
004_01	R₁R₁	Caucasian	1.12	1.05	Homozygous	RHD*01
004_02	R₁R₁	Caucasian	1.12	1.03	Homozygous	RHD*01
004_03	R₁R₁	Other	1.01	1.04	Homozygous	RHD*01
004_04	R₁R₁	Caucasian	1.07	1.03	Homozygous	RHD*01
004_05	R₁R₁	Caucasian	1.01	1.06	Homozygous	RHD*01
004_06	R₁R₁	Caucasian	0.99	1.04	Homozygous	RHD*01
004_07	R₁R₁	Caucasian	0.54	1.01	Discrepancy†	*RHD***01W.01*
004_08	R₁r	Caucasian	0.54	0.57	Hemizygous	RHD*01
004_09	R₁r	Caucasian	0.54	0.53	Hemizygous	RHD*01
004_10	R₁r	Chinese	0.51	0.56	Hemizygous	RHD*01
004_11	R₁r	Caucasian	0.53	0.54	Hemizygous	RHD*01
004_12	R₁r	Caucasian	0.55	0.52	Hemizygous	RHD*01
004_13	R₁r	Caucasian	0.54	0.6	Hemizygous	RHD*01
004_14	R₁r	Caucasian	1.06	0.99	Homozygous‡	*RHD**01
004_15	R₁r	Caucasian	0.53	0.50	Hemizygous	RHD*01W.01
004_16	R₁r	Caucasian	0.54	0.52	Hemizygous	RHD*01W.01
004_17	R₁r	Caucasian	0.58	0.56	Hemizygous	RHD*01W.01
004_18	R₁r	Caucasian	0.54	0.52	Hemizygous	RHD*01W.01
004_19	R₁r	Caucasian	0.54	0.47	Hemizygous	RHD*01W.01
004_20	R₁r	Caucasian	0.53	0.57	Hemizygous	RHD*01W.01
004_21	R₁r	Caucasian	0.52	0.57	Hemizygous	RHD*01W.01
004_22	R₁r	Caucasian	0.54	0.53	Hemizygous	RHD*01W.01
004_23	R₁r	Caucasian	0.52	0.53	Hemizygous	RHD*01W.01
004_24	R₁r	Caucasian	0.53	0.54	Hemizygous	RHD*01W.01
004_25	R₁r	Caucasian	0.53	0.51	Hemizygous	RHD*01W.01
004_26	R₁r	Caucasian	0.57	0.52	Hemizygous	RHD*01W.01
004_27	R₁r	Caucasian	0.56	0.54	Hemizygous	RHD*01W.01
004_28	R₁r	Caucasian	0.53	0.52	Hemizygous	RHD*01W.03
004_29	R₁R₂	Caucasian	1.09	1.03	Homozygous	RHD*01
004_30	R₁R₂	Caucasian	0.95	0.94	Homozygous	RHD*01
004_31	R₁R₂	Caucasian	1.08	1.05	Homozygous	RHD*01
004_32	R₁R₂	Caucasian	0.97	1.04	Homozygous	RHD*01
004_33	R₁R₂	Caucasian	1.03	1.08	Homozygous	RHD*01
004_34	R₁R₂	Caucasian	0.98	1.08	Homozygous	RHD*01
004_35	R₁R₂	Caucasian	0.46	0.51	Hemizygous§	*RHD***01W.02*
004_36	R₁R₂	Caucasian	0.51	0.51	Hemizygous§	*RHD**01
004_37	R₁R₂	Caucasian	0.53	0.49	Hemizygous§	*RHD**01
004_38	R₁R₂	Caucasian	0.51	0.53	Hemizygous§	*RHD**01
004_39	R₁R₂	Caucasian	0.52	0.51	Hemizygous§	*RHD**01
004_40	R₁R₂	Caucasian	0.53	0.52	Hemizygous§	*RHD**01
004_41	R₂R₂	Caucasian	1.01	0.99	Homozygous	RHD*01W.02
004_42	R₂R₂	Not disclosed	0.54	0.47	Hemizygous\|\|	*RHD***01W.02*
004_43	R₂R₂	Caucasian	1.01	1.02	Homozygous	RHD*01
004_44	R₂R₂	Caucasian	1.01	0.99	Homozygous	RHD*01
004_45	R₂R₂	Caucasian	1.03	1.02	Homozygous	RHD*01
004_46	R₂R₂	Caucasian	1.02	1.01	Homozygous	RHD*01
004_47	R₂R₂	Caucasian	1.01	1	Homozygous	RHD*01
004_48	R₂r	Caucasian	0.53	0.54	Hemizygous	RHD*01
004_49	R₂r	Caucasian	0.53	0.51	Hemizygous	RHD*01
004_50	R₂r	Caucasian	0.53	0.51	Hemizygous	RHD*01
004_51	R₂r	Caucasian	0.52	0.56	Hemizygous	RHD*01
004_52	R₂r	Caucasian	0.48	0.54	Hemizygous	RHD*01
004_53	R₂r	Caucasian	0.53	0.47	Hemizygous	RHD*01
004_54	R₂r	Caucasian	0.52	0.51	Hemizygous	RHD*01W.02
004_55	R₂r	Caucasian	0.53	0.52	Hemizygous	RHD*01W.02
004_56	R₂r	Caucasian	0.53	0.5	Hemizygous	RHD*01W.02
004_57	R₂r	Caucasian	0.51	0.57	Hemizygous	RHD*01W.02
004_58	R₂r	Caucasian	0.58	0.56	Hemizygous	RHD*01W.02
004_59	R₂r	Caucasian	0.51	0.53	Hemizygous	RHD*01W.02
004_60	R₂r	Caucasian	0.57	0.52	Hemizygous	RHD*01W.02
004_61	R₂r	Caucasian	0.55	0.53	Hemizygous	RHD*01W.02
004_62	R₂r	Caucasian	0.58	0.52	Hemizygous	RHD*01W.02
004_63	R₀r	Caucasian	0.46	0.46	Hemizygous	RHD*01
004_64	R₀r	Caucasian	0.5	0.53	Hemizygous	RHD*01
004_65	R₀r	Caucasian	0.46	0.49	Hemizygous	RHD*01
004_66	R₀r	Caucasian	0.49	0.52	Hemizygous	RHD*01
004_67	R₀r	Caucasian	0.53	0.51	Hemizygous	RHD*01
004_68	R₀r	Caucasian	0.52	0.51	Hemizygous	RHD*01
004_69	R₂R_Z	Caucasian	1.02	1.02	Homozygous	RHD*01
004_70- 004_123	R₁R₂	—	1.0¶	1.0¶	Homozygous	Not sequenced

The number of RHD copies per microliter present in a sample was compared with the reference gene AGO1 copies per microliter. If a sample presented a ratio of 1, it was considered homozygous; it was considered hemizygous when present with a ratio of 0.5. Bold in the table body represents incompatible results between predicted genotype by serology and dPCR.

—, individual ethnicities not given.

As supplied by the NHSBT, Bristol, United Kingdom.

†

Sample shows discrepancy between hemizygous RHD5 and homozygous RHD7 meaning that 1 of the RHD alleles has a deletion in exon 5.

Eight samples show incompatible dPCR results with serologically predicted genotypes indicating incorrectly predicted genotypes by serology; these samples include:

‡

R₁r sample shows the homozygous RHD gene.

6 R₁R₂ samples show the hemizygous RHD gene.

R₂R₂ sample shows the hemizygous RHD gene.

Average ratio.

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