Table 1. Sequence, exons covered,... | American Society of Hematology

Table 1.

Sequence, exons covered, product sizes, and annealing temperature for primers used for RHD LR-PCR

Primer name	Sequence 5′-3′	Exons	Size, bp	Annealing temperature, °C
RHD-1 forward	ATCCACTTTCCACCTCCCTGC	1	10 326	62
RHD-1 reverse	TCTTTGCACTTCTTCTGACAACA	1	10 326	62
RHD-2 forward	CTGGGAGAGTGAAGCTGGGTGTGA	2, 3	13 709	62
RHD-2 reverse	TTCATACACATCTCTACCCCCCCTC	2, 3	13 709	62
RHD-3 forward	GTTTGAGCCCAGGAGTTAGGGACCGAG	4	10 789	66
RHD-3 reverse	CCCACTGTGACCACCCAGCATTCTA	4	10 789	66
RHD-4 forward	CATACCTTTGAATTAAGCACTTCAC	5, 6, 7	9 895	66
RHD-4 reverse	CAGAATGGCCTTTACCAGCCAT	5, 6, 7	9 895	66
RHD-5 forward	GTTCAAGCTGTCAAGGAGACATCACTATACA	8	11 628	65
RHD-5 reverse	CCAGTTTTAAGAATTTGTCGGCCGGTCG	8	11 628	65
RHD-6 forward	ATACATTCCATCCAGAACTGTTCACC	9, 10	11 284	64
RHD-6 reverse	AGGCCAAGAGATCCTGGTGAAACTATCC	9, 10	11 284	64

Primer name	Sequence 5′-3′	Exons	Size, bp	Annealing temperature, °C
RHD-1 forward	ATCCACTTTCCACCTCCCTGC	1	10 326	62
RHD-1 reverse	TCTTTGCACTTCTTCTGACAACA	1	10 326	62
RHD-2 forward	CTGGGAGAGTGAAGCTGGGTGTGA	2, 3	13 709	62
RHD-2 reverse	TTCATACACATCTCTACCCCCCCTC	2, 3	13 709	62
RHD-3 forward	GTTTGAGCCCAGGAGTTAGGGACCGAG	4	10 789	66
RHD-3 reverse	CCCACTGTGACCACCCAGCATTCTA	4	10 789	66
RHD-4 forward	CATACCTTTGAATTAAGCACTTCAC	5, 6, 7	9 895	66
RHD-4 reverse	CAGAATGGCCTTTACCAGCCAT	5, 6, 7	9 895	66
RHD-5 forward	GTTCAAGCTGTCAAGGAGACATCACTATACA	8	11 628	65
RHD-5 reverse	CCAGTTTTAAGAATTTGTCGGCCGGTCG	8	11 628	65
RHD-6 forward	ATACATTCCATCCAGAACTGTTCACC	9, 10	11 284	64
RHD-6 reverse	AGGCCAAGAGATCCTGGTGAAACTATCC	9, 10	11 284	64