Effect of endostatin on the cell cycle profile of murine brain endothelial cells
| Culture conditions . | G1 . | S . | G2/M . | S/G1 . |
|---|---|---|---|---|
| No addition | 50.7 ± 2.8 | 16.7 ± 1.6 | 33.2 ± 3.3 | 0.33 ± 0.03 |
| Endostatin (1 μg/mL) | 52.5 ± 3.1 | 17.4 ± 1.0 | 30.6 ± 2.1 | 0.34 ± 0.04 |
| FGF-2 (5 ng/mL) | 47.1 ± 1.8 | 20.5 ± 2.6 | 32.6 ± 0.6 | 0.44 ± 0.07 |
| Endostatin + FGF-2 | 45.2 ± 1.3 | 16.6 ± 2.0* | 38.8 ± 1.1 | 0.37 ± 0.06* |
| FCS | 41.1 ± 4.7 | 21.4 ± 2.6 | 37.3 ± 1.9 | 0.54 ± 0.12 |
| Culture conditions . | G1 . | S . | G2/M . | S/G1 . |
|---|---|---|---|---|
| No addition | 50.7 ± 2.8 | 16.7 ± 1.6 | 33.2 ± 3.3 | 0.33 ± 0.03 |
| Endostatin (1 μg/mL) | 52.5 ± 3.1 | 17.4 ± 1.0 | 30.6 ± 2.1 | 0.34 ± 0.04 |
| FGF-2 (5 ng/mL) | 47.1 ± 1.8 | 20.5 ± 2.6 | 32.6 ± 0.6 | 0.44 ± 0.07 |
| Endostatin + FGF-2 | 45.2 ± 1.3 | 16.6 ± 2.0* | 38.8 ± 1.1 | 0.37 ± 0.06* |
| FCS | 41.1 ± 4.7 | 21.4 ± 2.6 | 37.3 ± 1.9 | 0.54 ± 0.12 |
FGF-2 = Fibroblast growth factor-2; FCS = fetal calf serum.
Murine brain endothelial (IBE) cells were cultured for 24 hours as indicated after which the percentages of cells in the different phases of the cell cycle were determined by flow cytometry. The S/G1 ratios are also given. Means ± SEM are given for 3 different experiments.
denotes P < .05, compared with the corresponding FGF-2 value.