Collagen and TPO stimulate formation of PI3,4,5P3 and PI3,4P2
| . | PI3,4,5P3 (cpm) . | PI3,4,P2 (cpm) . |
|---|---|---|
| Basal | 888 ± 200 | 736 ± 204 |
| Collagen (5 μg/mL) | 2640 ± 760 | 1968 ± 480 |
| Wortmannin (100 nmol/L) Collagen (5 μg/mL) | 248 ± 140 | 356 ± 76 |
| TPO (50 ng/mL) | 1612 ± 260 | 1400 ± 288 |
| Wortmannin (100 nmol/L) TPO (50 ng/mL) | 184 ± 140 | 320 ± 100 |
| . | PI3,4,5P3 (cpm) . | PI3,4,P2 (cpm) . |
|---|---|---|
| Basal | 888 ± 200 | 736 ± 204 |
| Collagen (5 μg/mL) | 2640 ± 760 | 1968 ± 480 |
| Wortmannin (100 nmol/L) Collagen (5 μg/mL) | 248 ± 140 | 356 ± 76 |
| TPO (50 ng/mL) | 1612 ± 260 | 1400 ± 288 |
| Wortmannin (100 nmol/L) TPO (50 ng/mL) | 184 ± 140 | 320 ± 100 |
[32P]-Labeled platelets were stimulated with collagen or TPO for 2 minutes. Phosphoinositides were extracted, deacylated, and analysed by high-performance liquid chromatography as described in “Materials and methods.” Indomethacin (10 μmol/L) was present throughout. Results are presented as mean ± SE for at least 5 experiments.