Representative experiment showing HbF reactivation, evaluated in terms of F cells and γ-chain content, in minibulk erythroid HPC cultures supplemented or not with graded NaB dosages ± saturating KL level
| Parameters . | Control . | NaB (0.1 mmol/L) . | NaB (0.2 mmol/L) . | NaB (0.5 mmol/L) . | KL (100 ng) . | KL + NaB (0.1 mmol/L) . | KL + NaB (0.2 mmol/L) . | KL + NaB (0.5 mmol/L) . |
|---|---|---|---|---|---|---|---|---|
| Mature erythroblasts (%) | 95 | 92 | 93 | 90 | 98 | 94 | 94 | 96 |
| F cells (%) | 0.8 | 13 | 20 | 26 | 23 | 52 | 57 | 63 |
| γ/γ + β (%) | 2.6 | 8.1 | 11.5 | 24.3 | 18.0 | 31.7 | 32.4 | 45.0 |
| Parameters . | Control . | NaB (0.1 mmol/L) . | NaB (0.2 mmol/L) . | NaB (0.5 mmol/L) . | KL (100 ng) . | KL + NaB (0.1 mmol/L) . | KL + NaB (0.2 mmol/L) . | KL + NaB (0.5 mmol/L) . |
|---|---|---|---|---|---|---|---|---|
| Mature erythroblasts (%) | 95 | 92 | 93 | 90 | 98 | 94 | 94 | 96 |
| F cells (%) | 0.8 | 13 | 20 | 26 | 23 | 52 | 57 | 63 |
| γ/γ + β (%) | 2.6 | 8.1 | 11.5 | 24.3 | 18.0 | 31.7 | 32.4 | 45.0 |