Presence of B progenitor cells in op/op bone marrow
| Bone marrow cells . | Frequency of B lineage cell generation3-150 . | No. of CFU–IL-7 per 105 cells . | ||
|---|---|---|---|---|
| +/? . | op/op . | +/? . | op/op . | |
| Freshly prepared3-151 | 1/237.8 ± 144.9 | 1/454.8 ± 156.23-152 | 48.3 ± 11.5 | 1.7 ± 2.93-153 |
| Cultured with PA6 and IL-73-155 | >1/6.5 | >1/6.5 | 26.7 ± 5.8 | 103.3 ± 18.93-153 |
| Bone marrow cells . | Frequency of B lineage cell generation3-150 . | No. of CFU–IL-7 per 105 cells . | ||
|---|---|---|---|---|
| +/? . | op/op . | +/? . | op/op . | |
| Freshly prepared3-151 | 1/237.8 ± 144.9 | 1/454.8 ± 156.23-152 | 48.3 ± 11.5 | 1.7 ± 2.93-153 |
| Cultured with PA6 and IL-73-155 | >1/6.5 | >1/6.5 | 26.7 ± 5.8 | 103.3 ± 18.93-153 |
The frequency of B lineage cell generation was measured by limiting dilution analysis.
Freshly prepared bone marrow cells (62.5, 125, 250, and 500 cells/well) from 8-week-old op/op and +/? mice were cultured on PA6 in the presence of 20-U/mL IL-7 for 8 days, and the number of wells containing B lineage cells was counted.
Significantly different from the corresponding normal mouse cultures (P < .05).
Significantly different from the corresponding normal mouse cultures (P < .002).
Cells cultured with PA6 and IL-7 for 2 weeks were harvested and recultured (by seeding 25, 50, 100, or 200 cells/well [48 wells/group]) with PA6 and IL-7 for 7 days.