Effect of Multiple Rounds of Transduction on Gene Transfer to Leukemia Cells With L-EGFP-SN
| Transduction Method . | % of Cells Expressing EGFP . | |||
|---|---|---|---|---|
| Nalm-6 . | Reh . | |||
| Fibronectin . | BSA . | Fibronectin . | BSA . | |
| 3 rounds, 1 day | 20.1 | 2.1 | 1.3 | 0.8 |
| 3 rounds, 3 days | 33.6 | 2.7 | 2.1 | 0.5 |
| Transduction Method . | % of Cells Expressing EGFP . | |||
|---|---|---|---|---|
| Nalm-6 . | Reh . | |||
| Fibronectin . | BSA . | Fibronectin . | BSA . | |
| 3 rounds, 1 day | 20.1 | 2.1 | 1.3 | 0.8 |
| 3 rounds, 3 days | 33.6 | 2.7 | 2.1 | 0.5 |
Fibronectin was used at 50 μg/mL and BSA was used at 2%.