Table 3. Ig Heavy Chain Gene Analysis of... | American Society of Hematology

Table 3.

Ig Heavy Chain Gene Analysis of PCBCL

Patient No.	Ig Isotype^3-150	VH Family	Closest Germline Gene	No. of Mutations	% of Homology	D Gene	JH Gene	Intraclonal Variation^3-151 (no. of clones sequenced)
1	γ	5	V5-51 (COS24)	15	95	NA	JH4b	0 (4)
2	γ	3	V3-7	29	90	NA	JH6c	0 (4)
3	μ, δ	3	V3-23	52	82	NA	JH4b	0 (5)
4	μ	3	V3-7	15	95	D21-9	JH3b	0.6 (8)
5	μ, δ	3	V3-30 (COS3)	17	94	NA	JH4b	1.4 (5)
6	μ, δ	4	V4-61 (3d279d)	35	88	DXP4	JH5b	2.6 (5)
7	γ	1	V1-2 (DP8)	37	87	NA	JH4b	3.4 (5)
8	α	1	V1-2 (DP8)	37	87	NA	JH4b	0.2 (5)

Patient No.	Ig Isotype^3-150	VH Family	Closest Germline Gene	No. of Mutations	% of Homology	D Gene	JH Gene	Intraclonal Variation^3-151 (no. of clones sequenced)
1	γ	5	V5-51 (COS24)	15	95	NA	JH4b	0 (4)
2	γ	3	V3-7	29	90	NA	JH6c	0 (4)
3	μ, δ	3	V3-23	52	82	NA	JH4b	0 (5)
4	μ	3	V3-7	15	95	D21-9	JH3b	0.6 (8)
5	μ, δ	3	V3-30 (COS3)	17	94	NA	JH4b	1.4 (5)
6	μ, δ	4	V4-61 (3d279d)	35	88	DXP4	JH5b	2.6 (5)
7	γ	1	V1-2 (DP8)	37	87	NA	JH4b	3.4 (5)
8	α	1	V1-2 (DP8)	37	87	NA	JH4b	0.2 (5)

Abbreviation: NA, the D gene could not definitely be assigned to a germline D gene.

Heavy chain isotype expression as determined by PCR.

The intraclonal variation is indicated as the number of mutations observed per clone, compared with the consensus sequence.