Table 1.

Summary of Single-Cell Assays

Medium +IL-6
No. of wells containing single cell at the start of culture  46  45  
No. of wells containing cells by the end of culture (28 days)  24  35  
 1-100 cells/well  20 0  
 100-200 cells/well  4  0  
 >500 cells/well 0  35  
No. of analyzed clones  4  14  
 No. of wells containing CD45+ cells  4  14 
 Percentage of CD45+ cells in analyzed clones (range)  0.9%-8.9%  29.9%-86.0% 
Medium +IL-6
No. of wells containing single cell at the start of culture  46  45  
No. of wells containing cells by the end of culture (28 days)  24  35  
 1-100 cells/well  20 0  
 100-200 cells/well  4  0  
 >500 cells/well 0  35  
No. of analyzed clones  4  14  
 No. of wells containing CD45+ cells  4  14 
 Percentage of CD45+ cells in analyzed clones (range)  0.9%-8.9%  29.9%-86.0% 
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