Table 2. Human NK Cell Precursor... | American Society of Hematology

Table 2.

Human NK Cell Precursor Frequency of Fresh CD34⁺ HPCs or 10-Day Culture of CD34⁺ HPCs in FL

Donor	Fresh CD34⁺ HPCs	10-Day Culture of CD34⁺ HPCs in FL
Donor	Fresh CD34⁺ HPCs	CD34^brightCD122⁺	CD34^dim/negCD122⁻
1	1:458	1:7	1:147
2	1:471	1:2	1:127
3	1:527	1:5	1:161

Donor	Fresh CD34⁺ HPCs	10-Day Culture of CD34⁺ HPCs in FL
Donor	Fresh CD34⁺ HPCs	CD34^brightCD122⁺	CD34^dim/negCD122⁻
1	1:458	1:7	1:147
2	1:471	1:2	1:127
3	1:527	1:5	1:161

Purified CD34⁺ HPCs were either assayed immediately for NK cell precursor frequency by LDA or further cultured in FL (100 ng/mL) for 10 days, sorted by FACS into CD34^brightCD122⁺ or CD34^dim/negCD122⁻ subsets (>98% pure), and then plated for LDA. In each LDA, cell populations were cultured in IL-15 (100 ng/mL) for an additional 14 days, stained with anti-CD56-PE MoAb, and analyzed for CD56 expression and NK cell precursor frequency, as described in Materials and Methods.

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