NKR Surface Expression by CD56+ Cells Derived From CD34+ HPCs Cultured in FL for 21 Days, Followed By Culture in IL-15 for 14 Days
| NKR . | NKR Subfamily . | % of NKR Expression in CD56+ Cells . | |||
|---|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 3 . | Mean ± SEM . | ||
| CD158a | KIR2DL1/S1 | 0.59 | 1.14 | 0.56 | 0.76 ± 0.2 |
| CD158b | KIR2DL2/L3/S2 | 7.03 | 3.10 | 6.80 | 5.6 ± 1.0 |
| p70/CDw159 | KIR3DL1/S1 | 0.88 | 0.37 | 0.04 | 0.43 ± 0.2 |
| p50.3 | KIR2DS4 | 5.1 | 0.08 | 0.10 | 1.76 ± 1.7 |
| NKG2A | C-type lectin | 80.72 | 47.8 | 76.42 | 68.3 ± 8.4 |
| NKR . | NKR Subfamily . | % of NKR Expression in CD56+ Cells . | |||
|---|---|---|---|---|---|
| Donor 1 . | Donor 2 . | Donor 3 . | Mean ± SEM . | ||
| CD158a | KIR2DL1/S1 | 0.59 | 1.14 | 0.56 | 0.76 ± 0.2 |
| CD158b | KIR2DL2/L3/S2 | 7.03 | 3.10 | 6.80 | 5.6 ± 1.0 |
| p70/CDw159 | KIR3DL1/S1 | 0.88 | 0.37 | 0.04 | 0.43 ± 0.2 |
| p50.3 | KIR2DS4 | 5.1 | 0.08 | 0.10 | 1.76 ± 1.7 |
| NKG2A | C-type lectin | 80.72 | 47.8 | 76.42 | 68.3 ± 8.4 |
Purified CD34+ HPCs were cultured in FL (100 ng/mL) for 21 days, washed, and cultured in IL-15 (100 ng/mL) for an additional 14 days. Cells were harvested and stained with anti-NKR MoAbs and anti-CD56-PE as described in Materials and Methods. The flow cytometry dot-plot for donor 1 is shown in Fig 2C. No detectable NKR or CD56 expression was observed in the 3-week cultures before the addition of IL-15. Similar NKR profiles were obtained when KL (100 ng/mL) was substituted for FL in these experiments (not shown). Individual results from 3 donors, as well as the mean ± SEM, are shown.