Effect of Anti-Integrin Antibodies on TEC Intracellular Ca2+
| Treatment . | Δ[Ca2+]in (nmol/L) (mean ± SE) . |
|---|---|
| M-Kid2 (anti-α3) | 54 ± 22 |
| MAR6 (anti-α6) | 71 ± 14 |
| MAR4 (anti-β1) | 87 ± 12 |
| 3E1 (anti-β4) | 74 ± 17 |
| B9.12.1 (anti–MHC-I) | 11 ± 5 |
| ATP (100 μmol/L) | 92 ± 18 |
| Treatment . | Δ[Ca2+]in (nmol/L) (mean ± SE) . |
|---|---|
| M-Kid2 (anti-α3) | 54 ± 22 |
| MAR6 (anti-α6) | 71 ± 14 |
| MAR4 (anti-β1) | 87 ± 12 |
| 3E1 (anti-β4) | 74 ± 17 |
| B9.12.1 (anti–MHC-I) | 11 ± 5 |
| ATP (100 μmol/L) | 92 ± 18 |
MoAbs or ATP were added on TEC monolayers loaded with Indo-1 as described in Materials and Methods. The level of [Ca2+]in was evaluated in the presence of 1 mmol/L CaCl2. Δ[Ca2+]in is the level of [Ca2+]in less the level determined in untreated cells. Mean values ± SE were calculated from three independent experiments.