Reconstitution in the Lymph Nodes of F1 Recipient Mice Engrafted With Adult BM or NBB Cells
| LN Cells from . | Percentage of Positive Cells ± SD . | |||
|---|---|---|---|---|
| Donor Origin3-150 . | CD4+ . | CD8+ . | Ig+ . | |
| Normal B10.D2 mice | 98.3 ± 2.5 | 35.2 ± 5.1 | 22.8 ± 2.9 | 37.4 ± 2.0 |
| Adult BM-engrafted F1 recipients | 88.9 ± 0.6 | 34.1 ± 7.9 | 17.5 ± 4.83-151 | 41.0 ± 2.8 |
| NBB-engrafted F1 recipients | ||||
| Day 0 | 82.8 ± 4.9 | 38.6 ± 10.2 | 17.8 ± 2.03-151 | 35.2 ± 9.6 |
| Day 2 | 87.9 ± 1.7 | 31.9 ± 2.7 | 17.0 ± 1.53-151 | 45.8 ± 3.3 |
| Day 5 | 83.4 ± 2.4 | 35.4 ± 4.9 | 15.8 ± 2.03-151 | 43.1 ± 3.4 |
| LN Cells from . | Percentage of Positive Cells ± SD . | |||
|---|---|---|---|---|
| Donor Origin3-150 . | CD4+ . | CD8+ . | Ig+ . | |
| Normal B10.D2 mice | 98.3 ± 2.5 | 35.2 ± 5.1 | 22.8 ± 2.9 | 37.4 ± 2.0 |
| Adult BM-engrafted F1 recipients | 88.9 ± 0.6 | 34.1 ± 7.9 | 17.5 ± 4.83-151 | 41.0 ± 2.8 |
| NBB-engrafted F1 recipients | ||||
| Day 0 | 82.8 ± 4.9 | 38.6 ± 10.2 | 17.8 ± 2.03-151 | 35.2 ± 9.6 |
| Day 2 | 87.9 ± 1.7 | 31.9 ± 2.7 | 17.0 ± 1.53-151 | 45.8 ± 3.3 |
| Day 5 | 83.4 ± 2.4 | 35.4 ± 4.9 | 15.8 ± 2.03-151 | 43.1 ± 3.4 |
LN cells from normal B10.D2 mice or from F1 recipients collected 2 to 3 months after grafting with B10.D2 adult BM cells or B10.D2 NBB cells taken on days 0, 2, and 5 after birth. Cells were labeled with PE-conjugated anti-CD4, FITC-conjugated anti-CD8 and FITC-conjugated GAM MoAbs. Values are means ± SD for 3 to 8 mice.
For chimerism evaluation, the percentage of cells of B10.D2 origin was estimated by the following calculation: 100-percentage of cells stained with anti–Lyt-1.1 MoAb specific of DBA/2 recipient origin. Anti–Lyt-1.1 MoAb was shown by FITC-conjugated GAM MoAb.
Significantly reduced compared with normal B10.D2 mice (Student’st test P < .05).