Proliferative Response of Cells From Newborn Blood or Adult Spleen, Peripheral Blood and BM to T-cell Mitogens and Allogeneic Cells
| Stimulation with . | Thymidine Incorporation Into Cells From Mean cpm ± SD (Stimulation Index) . | |||||
|---|---|---|---|---|---|---|
| Adult Spleen . | Adult Peripheral Blood . | Adult Bone Marrow . | Newborn Blood* . | |||
| Day 0 . | Day 2 . | Day 5 . | ||||
| — | 3355 ± 1318 | 1698 ± 846 | 5075 ± 3615 | 1494 ± 1154 | 5011 ± 1833 | 1622 ± 609 |
| PHA | 53973 ± 33611 (16.0) | 33233 ± 14898 (19.5) | 8423 ± 1412 (1.6) | 5984 ± 2036 (4.0) | 15879 ± 10363 (3.7) | 16609 ± 6893† (9.9) |
| Con A | 35663 ± 19945 (10.6) | 37873 ± 28716 (22.3) | 10730 ± 2831 (2.1) | 2533 ± 1466 (1.7) | 10227 ± 5636 (2.0) | 9352 ± 4806 (5.6) |
| Irradiated B10.D2 cells | 5887 ± 1882 | 4042 ± 2002 | 3911 ± 3103 | 2055 ± 1208 | 4483 ± 1214 | 2655 ± 569 |
| Irradiated C57Bl/6 cells | 25431 ± 5362 (4.3) | 14619 ± 17272 (3.6) | 8659 ± 2537 (2.2) | 4687 ± 2789 (1.6) | 9225 ± 3250 (2.0) | 10646 ± 2446† (4.0) |
| Stimulation with . | Thymidine Incorporation Into Cells From Mean cpm ± SD (Stimulation Index) . | |||||
|---|---|---|---|---|---|---|
| Adult Spleen . | Adult Peripheral Blood . | Adult Bone Marrow . | Newborn Blood* . | |||
| Day 0 . | Day 2 . | Day 5 . | ||||
| — | 3355 ± 1318 | 1698 ± 846 | 5075 ± 3615 | 1494 ± 1154 | 5011 ± 1833 | 1622 ± 609 |
| PHA | 53973 ± 33611 (16.0) | 33233 ± 14898 (19.5) | 8423 ± 1412 (1.6) | 5984 ± 2036 (4.0) | 15879 ± 10363 (3.7) | 16609 ± 6893† (9.9) |
| Con A | 35663 ± 19945 (10.6) | 37873 ± 28716 (22.3) | 10730 ± 2831 (2.1) | 2533 ± 1466 (1.7) | 10227 ± 5636 (2.0) | 9352 ± 4806 (5.6) |
| Irradiated B10.D2 cells | 5887 ± 1882 | 4042 ± 2002 | 3911 ± 3103 | 2055 ± 1208 | 4483 ± 1214 | 2655 ± 569 |
| Irradiated C57Bl/6 cells | 25431 ± 5362 (4.3) | 14619 ± 17272 (3.6) | 8659 ± 2537 (2.2) | 4687 ± 2789 (1.6) | 9225 ± 3250 (2.0) | 10646 ± 2446† (4.0) |
Values represent the mean ± SD of two to four tests for each experimental group. In each of the six individual experiments performed, the proliferative response of adult peripheral blood and/or adult BM and/or NBB were compared with the response of adult spleen.
Newborn blood was collected either on the day of birth (day 0) or 2 or 5 days later.
Significantly increased compared with newborn blood collected at day 0 (Student’s t test P < .05).