Effect of ATP11A on SCH6636-induced G2/M block
. | 24 h . | . | 36 h . | . | ||
|---|---|---|---|---|---|---|
| Transfected/treated . | G1, % . | G2/M, % . | G1, % . | G2/M, % . | ||
| pcDNA/DMSO | 61.5 | 18.6 | 58.3 | 23.2 | ||
| pcDNA/20 μM SCH66336 | 62.4 | 20.6 | 49.4 | 34.5 | ||
| ATP11A/DMSO | 53.7 | 31.5 | 62.0 | 22.2 | ||
| ATP11A/20 μM SCH66336 | 57.1 | 26.3 | 55.6 | 26.1 | ||
. | 24 h . | . | 36 h . | . | ||
|---|---|---|---|---|---|---|
| Transfected/treated . | G1, % . | G2/M, % . | G1, % . | G2/M, % . | ||
| pcDNA/DMSO | 61.5 | 18.6 | 58.3 | 23.2 | ||
| pcDNA/20 μM SCH66336 | 62.4 | 20.6 | 49.4 | 34.5 | ||
| ATP11A/DMSO | 53.7 | 31.5 | 62.0 | 22.2 | ||
| ATP11A/20 μM SCH66336 | 57.1 | 26.3 | 55.6 | 26.1 | ||
293 cells were transfected with either pcDNA plus EGFP or ATP11A plus EGFP. Twenty-four hours after transfection, treatment with either DMSO or 20 μM SCH66336 in DMSO was initiated. EGFP-positive cells were analyzed for DNA content using propidium iodide at the indicated time points. The percentage of cells in specific phases of the cell cycle is indicated.