Cell-cycle analysis of human Burkitt B-lymphoma cells treated with SP600125 inhibitor for 48 hours
Cell line . | Sub-G1 (apoptotic) . | G0/G1 . | S . | G2/M . |
|---|---|---|---|---|
| BJAB (medium) | 1 ± 0 | 42 ± 0 | 25 ± 0 | 32 ± 0 |
| BJAB (SP600125, 20 μM) | 3 ± 0 | 18 ± 1 | 15 ± 1 | 64 ± 2 |
| RAJI (medium) | 6 ± 1 | 39 ± 0 | 38 ± 1 | 16 ± 0 |
| RAJI (SP600125, 20 μM) | 13 ± 1 | 22 ± 1 | 30 ± 2 | 33 ± 2 |
Cell line . | Sub-G1 (apoptotic) . | G0/G1 . | S . | G2/M . |
|---|---|---|---|---|
| BJAB (medium) | 1 ± 0 | 42 ± 0 | 25 ± 0 | 32 ± 0 |
| BJAB (SP600125, 20 μM) | 3 ± 0 | 18 ± 1 | 15 ± 1 | 64 ± 2 |
| RAJI (medium) | 6 ± 1 | 39 ± 0 | 38 ± 1 | 16 ± 0 |
| RAJI (SP600125, 20 μM) | 13 ± 1 | 22 ± 1 | 30 ± 2 | 33 ± 2 |
Values presented are percentage of cells in each phase of cell cycle. Phase of cell cycle was determined by flow cytometric analysis of propidium iodide-stained cells. Results are presented as means ± SE of triplicate cultures. Experiments were performed 2 to 3 times with similar outcome.