Table 1.

Sequences of human-specific primers used for qRT-PCR

Human-specific genes
Forward primer, 5′ → 3′
Reverse primer, 5′ → 3′
Amplicon size, base pairs
Annealing temperature, °C
Actin   gca cag agc ctc gcc ttt   gga atc ctt ctg acc cat gc   213   59-65  
OCT4  gca gct cgg aag gca gat   tgg att tta aaa ggc aga aga ctt g   135   63  
CD31  gag tcc tgc tga ccc ttc tg   att ttg cac cgt cca gtc c   107   61  
CD34  tgg acc gcg ctt tgc t   ccc tgg gta ggt aac tct ggg   100   61  
CD45  cat ttg gct ttg cct ttc tg   ttc tct ttc aaa ggt gct tgc   167   61  
KDR/fik-1  cca gcc aag ctg tct cag t   ctg cat gtc agg ttg caa ag   272   61  
Flt-1  gac tag ata gcg tca cca gca g   gaa acc gtc aga atc ctc ctc   101   65  
VWF  ccc gaa agg cca ggt gta   agc aag ctt ccg ggg act   288   61-63  
VE-cadherin  gca gca gca ggt gct aac c   ttg ccc aca tat tct cct ttg   203   61  
SCL/TAL1  atg aga tgg aga tta ctg atg   gcc ccg ttc aca ttc tgc t   100   61  
LMO2  aac tgg gcc gga agc tct   ctt gaa aca ttc cag gtg ata ca   143   65  
EKLF  cgg aca cac agg atg act tc   ggc tgg tcc tca gac ttc ac   115   59-60  
PU.1  cac agc gag ttc gag agc tt   gat ggg tac tgg agg cac at   194   61  
GATA1  ggg atc aca ctg agc ttg c   acc cct gat tct ggt gtg g   176   64-65  
GATA2  gcg tct cca gcc tca tct t   gga aga gtc cgc tgc tgt ag   226   61  
c-myb  gtc aca aat tga ctg tta caa cac cat   ttc tac tag atg aga ggg tgt ctg agg   212   61  
CDX4  ctg tgg gcg gtg gaa cta   aaa cta cac gbvat act ttt ctt ttg tcc   186   61  
(HOXB4)*  gca cgg taa acc cca att a   ggc aac ttg tgg tct ttt tt   220   61-65  
AML 1a†  aag aca cag cac cct gga ga   gcc ttc ctc ata acg tgc at   151   60  
AML 1a/b‡  cgt gca cat aca tta gta gca ct acct ttg   ctt cca cga atc ttg ctt gca gag gtt aag   304   60  
AML 1c§  gaa gtc tga acc cag cat agt ggt cag cag   gtg gac gtc tct aga agg att cat tcc aag   231   60  
Epsilon  tgc tat taa aaa cat gga caa cc   gcc aga ata atc acc atc acg   126   61  
Zeta  att gtc tcc atg tgg gcc   tct gcg ggt ggc tga g   90   61-63  
Alpha  aag gtc ggc gcg cac gct   ctc agg tcg aac tgc ggg   101   61  
Gamma  gga caa ggc tac tat cac aag c   gga agt cag cac ctt ctt gc   193   63  
Beta
 		 ggc acc ttt gcc aca ctg
 		 cac tgg tgg ggt gaa ttc tt
 		 132
 		 61-63
 
Human-specific genes
Forward primer, 5′ → 3′
Reverse primer, 5′ → 3′
Amplicon size, base pairs
Annealing temperature, °C
Actin   gca cag agc ctc gcc ttt   gga atc ctt ctg acc cat gc   213   59-65  
OCT4  gca gct cgg aag gca gat   tgg att tta aaa ggc aga aga ctt g   135   63  
CD31  gag tcc tgc tga ccc ttc tg   att ttg cac cgt cca gtc c   107   61  
CD34  tgg acc gcg ctt tgc t   ccc tgg gta ggt aac tct ggg   100   61  
CD45  cat ttg gct ttg cct ttc tg   ttc tct ttc aaa ggt gct tgc   167   61  
KDR/fik-1  cca gcc aag ctg tct cag t   ctg cat gtc agg ttg caa ag   272   61  
Flt-1  gac tag ata gcg tca cca gca g   gaa acc gtc aga atc ctc ctc   101   65  
VWF  ccc gaa agg cca ggt gta   agc aag ctt ccg ggg act   288   61-63  
VE-cadherin  gca gca gca ggt gct aac c   ttg ccc aca tat tct cct ttg   203   61  
SCL/TAL1  atg aga tgg aga tta ctg atg   gcc ccg ttc aca ttc tgc t   100   61  
LMO2  aac tgg gcc gga agc tct   ctt gaa aca ttc cag gtg ata ca   143   65  
EKLF  cgg aca cac agg atg act tc   ggc tgg tcc tca gac ttc ac   115   59-60  
PU.1  cac agc gag ttc gag agc tt   gat ggg tac tgg agg cac at   194   61  
GATA1  ggg atc aca ctg agc ttg c   acc cct gat tct ggt gtg g   176   64-65  
GATA2  gcg tct cca gcc tca tct t   gga aga gtc cgc tgc tgt ag   226   61  
c-myb  gtc aca aat tga ctg tta caa cac cat   ttc tac tag atg aga ggg tgt ctg agg   212   61  
CDX4  ctg tgg gcg gtg gaa cta   aaa cta cac gbvat act ttt ctt ttg tcc   186   61  
(HOXB4)*  gca cgg taa acc cca att a   ggc aac ttg tgg tct ttt tt   220   61-65  
AML 1a†  aag aca cag cac cct gga ga   gcc ttc ctc ata acg tgc at   151   60  
AML 1a/b‡  cgt gca cat aca tta gta gca ct acct ttg   ctt cca cga atc ttg ctt gca gag gtt aag   304   60  
AML 1c§  gaa gtc tga acc cag cat agt ggt cag cag   gtg gac gtc tct aga agg att cat tcc aag   231   60  
Epsilon  tgc tat taa aaa cat gga caa cc   gcc aga ata atc acc atc acg   126   61  
Zeta  att gtc tcc atg tgg gcc   tct gcg ggt ggc tga g   90   61-63  
Alpha  aag gtc ggc gcg cac gct   ctc agg tcg aac tgc ggg   101   61  
Gamma  gga caa ggc tac tat cac aag c   gga agt cag cac ctt ctt gc   193   63  
Beta
 		 ggc acc ttt gcc aca ctg
 		 cac tgg tgg ggt gaa ttc tt
 		 132
 		 61-63
 
*

These primers do not distinguish between human and murine HOXB4.

†

These primers amplify an amplicon in exon 7A of the AML 1 gene that is specific for the a isoform of AML 1.50 

‡

These primers amplify an amplicon in exon 3 of the AML 1 gene that is specific for both the a and b isoforms of AML 1.50 

§

These primers amplify an amplicon that spans exon 1 and 2 of the AML 1 gene and is specific for the c isoform of AML 1.50 

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