Relative cell viability, by I3C concentration
Patient no. . | Relative cell viability, % . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | 0 μM I3C . | 1 μM I3C . | 2 μM I3C . | 5 μM I3C . | 10 μM I3C . | 20 μM I3C . | 30 μM I3C . | ||||||
| 1 | 100 ± 4.2 | 103 ± 2.0 | 104 ± 1.2 | 103 ± 4.6 | 102 ± 5.3 | 80 ± 3.1 | 53 ± 0.0 | ||||||
| 2 | 100 ± 5.7 | 106 ± 7.2 | 104 ± 6.4 | 86 ± 3.3 | 88 ± 2.8 | 57 ± 6.7 | 60 ± 5.7 | ||||||
| 3 | 100 ± 7.0 | 102 ± 8.3 | 95 ± 1.6 | 98 ± 3.6 | 89 ± 1.2 | 86 ± 1.2 | 63 ± 3.6 | ||||||
| 4 | 100 ± 2.7 | 85 ± 3.4 | 78 ± 1.6 | 80 ± 5.0 | 76 ± 4.3 | 63 ± 1.2 | 51 ± 2.5 | ||||||
| 5 | 100 ± 2.4 | 94 ± 1.1 | 95 ± 2.2 | 88 ± 0.3 | 85 ± 1.1 | 78 ± 1.5 | 62 ± 1.9 | ||||||
| 6 | 100 ± 4.1 | 92 ± 0.2 | 91 ± 0.2 | 90 ± 3.2 | 80 ± 3.5 | 68 ± 0.9 | 52 ± 1.5 | ||||||
| 7 | 100 ± 6.1 | 112 ± 7.7 | 105 ± 6.8 | 108 ± 3.6 | 98 ± 3.0 | 95 ± 7.1 | 69 ± 6.1 | ||||||
| 8 | 100 ± 3.0 | 95 ± 3.7 | 88 ± 1.8 | 90 ± 5.6 | 85 ± 4.8 | 71 ± 1.3 | 57 ± 2.7 | ||||||
| 9 | 100 ± 6.1 | 92 ± 6.1 | 85 ± 3.4 | 74 ± 5.4 | 63 ± 3.1 | 68 ± 1.5 | 51 ± 6.1 | ||||||
| 10 | 100 ± 4.9 | 103 ± 4.5 | 105 ± 7.3 | 99 ± 2.2 | 91 ± 1.6 | 83 ± 4.9 | 47 ± 2.1 | ||||||
Patient no. . | Relative cell viability, % . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | 0 μM I3C . | 1 μM I3C . | 2 μM I3C . | 5 μM I3C . | 10 μM I3C . | 20 μM I3C . | 30 μM I3C . | ||||||
| 1 | 100 ± 4.2 | 103 ± 2.0 | 104 ± 1.2 | 103 ± 4.6 | 102 ± 5.3 | 80 ± 3.1 | 53 ± 0.0 | ||||||
| 2 | 100 ± 5.7 | 106 ± 7.2 | 104 ± 6.4 | 86 ± 3.3 | 88 ± 2.8 | 57 ± 6.7 | 60 ± 5.7 | ||||||
| 3 | 100 ± 7.0 | 102 ± 8.3 | 95 ± 1.6 | 98 ± 3.6 | 89 ± 1.2 | 86 ± 1.2 | 63 ± 3.6 | ||||||
| 4 | 100 ± 2.7 | 85 ± 3.4 | 78 ± 1.6 | 80 ± 5.0 | 76 ± 4.3 | 63 ± 1.2 | 51 ± 2.5 | ||||||
| 5 | 100 ± 2.4 | 94 ± 1.1 | 95 ± 2.2 | 88 ± 0.3 | 85 ± 1.1 | 78 ± 1.5 | 62 ± 1.9 | ||||||
| 6 | 100 ± 4.1 | 92 ± 0.2 | 91 ± 0.2 | 90 ± 3.2 | 80 ± 3.5 | 68 ± 0.9 | 52 ± 1.5 | ||||||
| 7 | 100 ± 6.1 | 112 ± 7.7 | 105 ± 6.8 | 108 ± 3.6 | 98 ± 3.0 | 95 ± 7.1 | 69 ± 6.1 | ||||||
| 8 | 100 ± 3.0 | 95 ± 3.7 | 88 ± 1.8 | 90 ± 5.6 | 85 ± 4.8 | 71 ± 1.3 | 57 ± 2.7 | ||||||
| 9 | 100 ± 6.1 | 92 ± 6.1 | 85 ± 3.4 | 74 ± 5.4 | 63 ± 3.1 | 68 ± 1.5 | 51 ± 6.1 | ||||||
| 10 | 100 ± 4.9 | 103 ± 4.5 | 105 ± 7.3 | 99 ± 2.2 | 91 ± 1.6 | 83 ± 4.9 | 47 ± 2.1 | ||||||
A total of 1 × 105 cells in RPMI 1640 were seeded in a 96-well plate, treated with the indicated concentrations of I3C for 72 hours, and then analyzed for cell viability by the MTT method.