Impact of anti-JAM-A antibody BV11 on leukocyte recruitment
. | I/R and BV11, mean ± SEM . | . | I/R and IgG2b, mean ± SEM . | . | ||
|---|---|---|---|---|---|---|
. | 30 min . | 120 min . | 30 min . | 120 min . | ||
| Leukocyte rolling in postsinusoidal venules, cells/mm/s | 9.6 ± 0.9 | 9.0 ± 2.1 | 10.9 ± 1.4 | 6.9 ± 2.4 | ||
| Leukocyte adherence in postsinusoidal venules, cells/mm2 | 302.7 ± 32.3 | 285.0 ± 45.1 | 284.5 ± 28.8 | 263.8 ± 28.4 | ||
| Leukocyte accumulation in sinusoids, cells/acinus | 10.6 ± 1.0 | 12.7 ± 1.5 | 11.2 ± 0.7 | 12.6 ± 1.0 | ||
. | I/R and BV11, mean ± SEM . | . | I/R and IgG2b, mean ± SEM . | . | ||
|---|---|---|---|---|---|---|
. | 30 min . | 120 min . | 30 min . | 120 min . | ||
| Leukocyte rolling in postsinusoidal venules, cells/mm/s | 9.6 ± 0.9 | 9.0 ± 2.1 | 10.9 ± 1.4 | 6.9 ± 2.4 | ||
| Leukocyte adherence in postsinusoidal venules, cells/mm2 | 302.7 ± 32.3 | 285.0 ± 45.1 | 284.5 ± 28.8 | 263.8 ± 28.4 | ||
| Leukocyte accumulation in sinusoids, cells/acinus | 10.6 ± 1.0 | 12.7 ± 1.5 | 11.2 ± 0.7 | 12.6 ± 1.0 | ||
The impact of either BV11 antibody or isotype-matched control rat IgG2b on leukocyte rolling adherence/accumulation was analyzed using intravital microscopy in hepatic microvessels after 90 minutes of ischemia followed by 30 and 120 minutes of reperfusion. Transmigrated CD45+ leukocytes were counted in tissue sections (reperfusion: 140 minutes). n = 6 each group; mean ± SEM. Leukocyte transmigration of extravascular CD45+ cells/mm2 at 140 minutes was 89.0 ± 8.9 (P <.05 versus IgG2b) for I/R plus BV11 and 213.2 ± 23.5 for I/R plus IgG2b.