Nucleotide sequences of primer sets used in the study
. | |Primer sets| . | . | |
|---|---|---|---|
| Genes . | Reverse . | Forward . | |
| HAS1 | 5′VIC-GGGCTTGTCAGAGCTACTT | 5′AGGGCGTCTCTGAGTAGCAG | |
| HAS2 | 5′VIC-CCTCATCTGTGGAGATGGGT | 5′TCCCAGAGGTCCACTAATGC | |
| HAS3 | 5′VIC-CATCCAGGTGTGCGACTCTG | 5′CGCTGCTCAGGAAGGAAATC | |
| CD19 | 5′FAM-TACTATGGCACTGGCTGCTG | 5′CACGTTCCCGTACTGGTTCT | |
| RHAMM | 5′FAM-TGACAAAGATACTACCTTGCCTGCT | 5′CAGCATTTAGCCTTGCTTCCATC | |
| HAS1 cloning primers | 5′GCCTTCGCCCTGCTCATCCTG | 5′GTAGAACAGACGCAGCACA | |
. | |Primer sets| . | . | |
|---|---|---|---|
| Genes . | Reverse . | Forward . | |
| HAS1 | 5′VIC-GGGCTTGTCAGAGCTACTT | 5′AGGGCGTCTCTGAGTAGCAG | |
| HAS2 | 5′VIC-CCTCATCTGTGGAGATGGGT | 5′TCCCAGAGGTCCACTAATGC | |
| HAS3 | 5′VIC-CATCCAGGTGTGCGACTCTG | 5′CGCTGCTCAGGAAGGAAATC | |
| CD19 | 5′FAM-TACTATGGCACTGGCTGCTG | 5′CACGTTCCCGTACTGGTTCT | |
| RHAMM | 5′FAM-TGACAAAGATACTACCTTGCCTGCT | 5′CAGCATTTAGCCTTGCTTCCATC | |
| HAS1 cloning primers | 5′GCCTTCGCCCTGCTCATCCTG | 5′GTAGAACAGACGCAGCACA | |
For these primer sets, the reverse primers were labeled at their 5 ends with 6-carboxyflourescein (FAM) or VIC (chemical name not disclosed; ABI). Primers were designed using the “Primer 3” or “Gene Tool” programs based on the published cDNA sequences of the HASs, CD19, and RHAMM.