Progenitor cell content of c-MybPlt3/Plt3 and c-MybPlt4/Plt4 spleens
. | No. of colonies per 5 × 104 spleen cells . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse . | Blast . | G . | GM . | M . | Eo . | Meg . | |||||
| Mpl-/- c-MybPlt3/Plt3 | |||||||||||
| IL-3 | 4 ± 1 | 4 ± 1 | 3 ± 4 | 3 ± 1 | 0 | 72 ± 76 | |||||
| S3E | 3 ± 4 | 4 ± 3 | 3 ± 3 | 4 ± 7 | 0 | 91 ± 105 | |||||
| Mpl-/- c-MybPlt4/Plt4 | |||||||||||
| IL-3 | 4 ± 3 | 11 ± 6 | 6 ± 2 | 7 ± 1 | 0 | 70 ± 28 | |||||
| S3E | 5 ± 4 | 12 ± 5 | 7 ± 5 | 5 ± 4 | 0 | 100 ± 23 | |||||
| Mpl-/- c-Myb+/+ | |||||||||||
| IL-3 | 0.2 ± 0.3 | 0.3 ± 0.3 | 0 | 0 | 0 | 0.6 ± 0.4 | |||||
| S3E | 0.1 ± 0.2 | 0.4 ± 0.5 | 0.5 ± 0.8 | 0.4 ± 0.8 | 0.1 ± 0.4 | 4 ± 5 | |||||
| Mpl+/+ c-Myb+/+ | |||||||||||
| IL-3 | 0.9 ± 1.0 | 0.5 ± 0.4 | 0.1 ± 0.2 | 0.1 ± 0.2 | 0 | 1.1 ± 0.9 | |||||
| S3E | 1 ± 1 | 0.6 ± 0.8 | 0.2 ± 0.4 | 0.2 ± 0.4 | 0 | 7 ± 7 | |||||
| Mpl+/+ c-MybPlt4/Plt4 | |||||||||||
| IL-3 | 4.5 ± 0.7 | 14 ± 11 | 11.0 ± 6.3 | 9.5 ± 2.1 | 0 | 84 ± 49 | |||||
| S3E | 2.0 ± 1.4 | 8.0 ± 1.4 | 12.0 ± 5.7 | 15.0 ± 7.8 | 0 | 126 ± 14 | |||||
. | No. of colonies per 5 × 104 spleen cells . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse . | Blast . | G . | GM . | M . | Eo . | Meg . | |||||
| Mpl-/- c-MybPlt3/Plt3 | |||||||||||
| IL-3 | 4 ± 1 | 4 ± 1 | 3 ± 4 | 3 ± 1 | 0 | 72 ± 76 | |||||
| S3E | 3 ± 4 | 4 ± 3 | 3 ± 3 | 4 ± 7 | 0 | 91 ± 105 | |||||
| Mpl-/- c-MybPlt4/Plt4 | |||||||||||
| IL-3 | 4 ± 3 | 11 ± 6 | 6 ± 2 | 7 ± 1 | 0 | 70 ± 28 | |||||
| S3E | 5 ± 4 | 12 ± 5 | 7 ± 5 | 5 ± 4 | 0 | 100 ± 23 | |||||
| Mpl-/- c-Myb+/+ | |||||||||||
| IL-3 | 0.2 ± 0.3 | 0.3 ± 0.3 | 0 | 0 | 0 | 0.6 ± 0.4 | |||||
| S3E | 0.1 ± 0.2 | 0.4 ± 0.5 | 0.5 ± 0.8 | 0.4 ± 0.8 | 0.1 ± 0.4 | 4 ± 5 | |||||
| Mpl+/+ c-Myb+/+ | |||||||||||
| IL-3 | 0.9 ± 1.0 | 0.5 ± 0.4 | 0.1 ± 0.2 | 0.1 ± 0.2 | 0 | 1.1 ± 0.9 | |||||
| S3E | 1 ± 1 | 0.6 ± 0.8 | 0.2 ± 0.4 | 0.2 ± 0.4 | 0 | 7 ± 7 | |||||
| Mpl+/+ c-MybPlt4/Plt4 | |||||||||||
| IL-3 | 4.5 ± 0.7 | 14 ± 11 | 11.0 ± 6.3 | 9.5 ± 2.1 | 0 | 84 ± 49 | |||||
| S3E | 2.0 ± 1.4 | 8.0 ± 1.4 | 12.0 ± 5.7 | 15.0 ± 7.8 | 0 | 126 ± 14 | |||||
Data are the mean ± standard deviation from analysis of 2 to 7 mice of each genotype.
G indicates granulocyte; GM, granulocyte-macrophage; M, macrophage; Eo, eosinophil, Meg, megakaryocyte; S3E, SCF + IL-3 + EPO.