V_H and V_L from 38C13 were fused with the leader sequence of LAMP1 (Sig), the marker HA1, and the lysosomal targeting signal of LAMP1 (LAMP1) by strand overlapping PCR. Constructs without LAMP1 signals were prepared and used as controls. Extension was done at 72°C for 60 seconds for each construct.

Temp indicates temperature; sig, 5′ sorting signal of the mouse LAMP1; HA1, tag-peptide derived from hemagglutinin of influenza virus; V_H, variable region of the heavy chain of the Id expressed by the 38C13 lymphoma; V_L, variable region of the light chain of the Id expressed by the 38C13 lymphoma; LAMP1, 3′ transmembrane and cytoplasmic tail of the mouse LAMP1.

Sense and antisense primers are shown. Restriction enzyme sites are underlined