Table 3. Quantitative RT-PCR results... | American Society of Hematology

Table 3.

Quantitative RT-PCR results from purified patient samples

	TACC3	FGFR3	LETM1	MMSET total	MMSET III	MMSET I	MMSET II	RE-IIBP	WHSC2
Patient no.
1335	1.34	0.56	8.36	4.66	10.08	11.13	3.24	11.39	11.06
1336	0.32	0.04	1.84	0.59	2.40	0.89	0.40	0.82	0.97
1677^*	0.33	0.04	1.54	1.04	2.17	2.49	0.90	0.93	0.95
1676^*	0.61	0.05	2.56	0.92	2.09	1.84	1.13	2.31	2.19
1668^*	0.19	0.01	1.19	0.61	0.96	0.94	0.68	0.29	1.03
1644^*	1.80	0.55	6.66	17.6	5.18	1.54	2.91	6.74	6.41
1645^*	5.37	8.25	5.69	1.82	2.76	0.81	1.40	3.47	4.51
1364	0.95	0.05	4.04	1.72	2.08	2.27	1.39	2.92	2.49
1243	0.55	0.05	3.53	1.14	2.83	0.71	0.85	1.53	2.72
527	1.03	0.01	1.20	0.71	1.63	0.68	0.55	0.53	1.30
1660^*	0.42	0.01	1.56	0.99	1.93	1.09	0.73	1.08	1.18
1504 [MB4-1, NEG]	18.78	1.22	0.48	77.81	77.29	167.65	29.73	286.73	0.89
1560^* [MB4-1, NEG]	0.76	0.63	1.65	24.53	71.95	72.58	17.21	34.77	1.25
1649^* [MB4-1, POS]	0.74	339.10	4.49	22.13	95.90	14.02	22.84	140.61	2.97
1237 [MB4-1, POS]	2.54	931.97	11.48	71.59	158.23	42.47	43.93	156.32	4.56
1308 [MB4-3, NEG]	0.23	0.07	1.58	45.25	1.85	70.20	34.78	124.79	2.33
1661 [MB4-3, POS]	0.21	58.10	0.90	12.47	0.65	27.86	9.60	24.48	0.79
1661 [MB4-3, POS]	4.02	139.80	3.50	40.50	1.40	30.13	31.41	38.23	2.14
Totals
t(4;14)^NEG mean
REL (mean ΔCt)	1.17 (8.63)	0.87 (12.6)	3.47 (4.91)	2.89 (7.49)	3.10 (10.4)	2.22 (9.81)	1.29 (4.78)	2.91 (10.1)	3.16 (5.67)
t(4;14)^POS mean
REL (mean ΔCt)	3.90 (7.84)	210 (4.90)	3.44 (5.28)	42.0 (2.91)	58.2 (7.82)	60.7 (4.84)	27.1 (0.23)	115 (4.48)	2.13 (5.99)
ttest	.182	.054	.904	< .001	.005	.001	< .001	.001	.497

	TACC3	FGFR3	LETM1	MMSET total	MMSET III	MMSET I	MMSET II	RE-IIBP	WHSC2
Patient no.
1335	1.34	0.56	8.36	4.66	10.08	11.13	3.24	11.39	11.06
1336	0.32	0.04	1.84	0.59	2.40	0.89	0.40	0.82	0.97
1677^*	0.33	0.04	1.54	1.04	2.17	2.49	0.90	0.93	0.95
1676^*	0.61	0.05	2.56	0.92	2.09	1.84	1.13	2.31	2.19
1668^*	0.19	0.01	1.19	0.61	0.96	0.94	0.68	0.29	1.03
1644^*	1.80	0.55	6.66	17.6	5.18	1.54	2.91	6.74	6.41
1645^*	5.37	8.25	5.69	1.82	2.76	0.81	1.40	3.47	4.51
1364	0.95	0.05	4.04	1.72	2.08	2.27	1.39	2.92	2.49
1243	0.55	0.05	3.53	1.14	2.83	0.71	0.85	1.53	2.72
527	1.03	0.01	1.20	0.71	1.63	0.68	0.55	0.53	1.30
1660^*	0.42	0.01	1.56	0.99	1.93	1.09	0.73	1.08	1.18
1504 [MB4-1, NEG]	18.78	1.22	0.48	77.81	77.29	167.65	29.73	286.73	0.89
1560^* [MB4-1, NEG]	0.76	0.63	1.65	24.53	71.95	72.58	17.21	34.77	1.25
1649^* [MB4-1, POS]	0.74	339.10	4.49	22.13	95.90	14.02	22.84	140.61	2.97
1237 [MB4-1, POS]	2.54	931.97	11.48	71.59	158.23	42.47	43.93	156.32	4.56
1308 [MB4-3, NEG]	0.23	0.07	1.58	45.25	1.85	70.20	34.78	124.79	2.33
1661 [MB4-3, POS]	0.21	58.10	0.90	12.47	0.65	27.86	9.60	24.48	0.79
1661 [MB4-3, POS]	4.02	139.80	3.50	40.50	1.40	30.13	31.41	38.23	2.14
Totals
t(4;14)^NEG mean
REL (mean ΔCt)	1.17 (8.63)	0.87 (12.6)	3.47 (4.91)	2.89 (7.49)	3.10 (10.4)	2.22 (9.81)	1.29 (4.78)	2.91 (10.1)	3.16 (5.67)
t(4;14)^POS mean
REL (mean ΔCt)	3.90 (7.84)	210 (4.90)	3.44 (5.28)	42.0 (2.91)	58.2 (7.82)	60.7 (4.84)	27.1 (0.23)	115 (4.48)	2.13 (5.99)
ttest	.182	.054	.904	< .001	.005	.001	< .001	.001	.497

The relative expression level (REL) was determined by the ΔΔCt analysis method using the Raji cell line as a reference expression level of 1 for all reactions except FGFR3. Since Raji does not express FGFR3, we used the KMS-18 myeloma cell line as a reference expression level of 100, as this t(4;14)^POS cell line had the highest expression level.

For t(4;14)^POS samples, the breakpoint type and FGFR3 expression status are noted below the patient identifier. The mean ΔCt (Ct^Target - Ct^GAPDH) is shown to indicate the differences in absolute expression levels between each gene (the lower the ΔCt value the greater the expression level).

Patient samples represent diagnostic samples

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