Stages of neutrophil differentiation in CD34+ cells transduced with eGFP or Id2
. | eGFP . | . | Id2 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I and II | 43.9 ± 11.5 | 26.5 ± 6.7 | 32.8 ± 6.7 | 19.7 ± 5.1 | ||
| Myelocyte | 24.7 ± 7.9 | 15.9 ± 7.7 | 16.8 ± 6.4 | 17.5 ± 6.4 | ||
| Metamyelocyte/banded | 25.7 ± 9.7 | 46.4 ± 6.2 | 35.7 ± 8.4 | 47.9 ± 4.2 | ||
| Segmented | 2.7 ± 1.9 | 8.6 ± 2.1 | 8.5 ± 5.4 | 13.4 ± 4.8 | ||
. | eGFP . | . | Id2 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I and II | 43.9 ± 11.5 | 26.5 ± 6.7 | 32.8 ± 6.7 | 19.7 ± 5.1 | ||
| Myelocyte | 24.7 ± 7.9 | 15.9 ± 7.7 | 16.8 ± 6.4 | 17.5 ± 6.4 | ||
| Metamyelocyte/banded | 25.7 ± 9.7 | 46.4 ± 6.2 | 35.7 ± 8.4 | 47.9 ± 4.2 | ||
| Segmented | 2.7 ± 1.9 | 8.6 ± 2.1 | 8.5 ± 5.4 | 13.4 ± 4.8 | ||
After 14 and 17 days of differentiation, transduced cells were separated from the nontransduced cells, and cytospins were made. Cytospins were stained with May-Grünwald-Giemsa solution. Neutrophil differentiation was expressed as a percentage of myeloblast, promyelocyte I, promyelocyte II, myelocyte, and metamyelocyte toward neutrophils with banded and segmented nuclei within the neutrophil lineage. Data are expressed as an average of 5 independent experiments plus or minus SEM.