Table 2.

Effect of 8-Cl-Ado on RNA transcripts of apoptotic proteins

% of control
MCL-1
BCL-2
Patient no.
4 h
24 h
4 h
24 h
15   72   49   ND   ND  
16   62   59   92   114  
17   81   64   107   49  
18   143   118   108   152  
19   113   129   80   118  
20   89   98   98   105  
21   4*  0   ND   ND  
22
 		 68*
 		 0
 		 ND
 		 ND
 
% of control
MCL-1
BCL-2
Patient no.
4 h
24 h
4 h
24 h
15   72   49   ND   ND  
16   62   59   92   114  
17   81   64   107   49  
18   143   118   108   152  
19   113   129   80   118  
20   89   98   98   105  
21   4*  0   ND   ND  
22
 		 68*
 		 0
 		 ND
 		 ND
 

Total RNA was isolated from CLL lymphocytes (1 × 107/mL) after treatment with 10 μM 8-Cl-Ado at the indicated times. Real-time RT-PCR experiments were carried out to measure the RNA transcripts of antiapoptotic proteins MCL-1 and BCL-2. The values are expressed as percentage of control (untreated) cells.

ND indicates not done.

*

These values were measured at 18 hours in poly(A)-enriched RNA rather than total RNA

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