RT-PCR primers used for quantification of putative cytoprotective NF-κB target genes
Genes . | Forward primers . | Reverse primers . |
|---|---|---|
| hA20 | 5′–aga tca tcc aca aag ccc tca tc –3′ | 5′–aat tgc cgt cac cgt tcg t–3′ |
| hXAIP1 | 5′–ggt gtt ttc tca gta gtt ctt acc aga ca–3′ | 5′–atg cta aat ggt atc cag ggt gc–3′ |
| Survivin | 5′–acc agg tga gaa gtg agg ga–3′ | 5′–aac agt aga gga gcc agg ga–3′ |
| Bcl-XL | 5′–gaa cgg cgg ctg gga t–3′ | 5′–agc ggt tga agc gtt cct g–3′ |
| Bfl1/A1 | 5′–aca cag gag aat gga taa ggc aaa–3′ | 5′–agt cat cca gcc aga ttt agg ttc–3′ |
| cFLIP | 5′–gtg gag acc cac ctg ctc a–3′ | 5′–gga cac atc aga ttt atc caa atc c–3′ |
| API1 | 5′–gcc ttt ctc caa acc ctc tt–3′ | 5′–cat tcg agc tgc atg tgt ct–3′ |
| API2 | 5′–taa tac cgg gaa cat gaa g–3′ | 5′–gga cac tga tca aaa aag ac–3′ |
| Bcl2 | 5′–agg aag tga aca ttt cgg tga c–3′ | 5′–gct cag ttc cag gac cag g–3′ |
| Gadd45β | 5′–tgg aag agc tcg tgg cgt g–3′ | 5′–cct cgt caa tgg aga ggc–3′ |
| MALT1 | 5′–gga tct tac tga ata tga gat gc–3′ | 5′–gac aat ttt cag acc tat atg g–3′ |
Genes . | Forward primers . | Reverse primers . |
|---|---|---|
| hA20 | 5′–aga tca tcc aca aag ccc tca tc –3′ | 5′–aat tgc cgt cac cgt tcg t–3′ |
| hXAIP1 | 5′–ggt gtt ttc tca gta gtt ctt acc aga ca–3′ | 5′–atg cta aat ggt atc cag ggt gc–3′ |
| Survivin | 5′–acc agg tga gaa gtg agg ga–3′ | 5′–aac agt aga gga gcc agg ga–3′ |
| Bcl-XL | 5′–gaa cgg cgg ctg gga t–3′ | 5′–agc ggt tga agc gtt cct g–3′ |
| Bfl1/A1 | 5′–aca cag gag aat gga taa ggc aaa–3′ | 5′–agt cat cca gcc aga ttt agg ttc–3′ |
| cFLIP | 5′–gtg gag acc cac ctg ctc a–3′ | 5′–gga cac atc aga ttt atc caa atc c–3′ |
| API1 | 5′–gcc ttt ctc caa acc ctc tt–3′ | 5′–cat tcg agc tgc atg tgt ct–3′ |
| API2 | 5′–taa tac cgg gaa cat gaa g–3′ | 5′–gga cac tga tca aaa aag ac–3′ |
| Bcl2 | 5′–agg aag tga aca ttt cgg tga c–3′ | 5′–gct cag ttc cag gac cag g–3′ |
| Gadd45β | 5′–tgg aag agc tcg tgg cgt g–3′ | 5′–cct cgt caa tgg aga ggc–3′ |
| MALT1 | 5′–gga tct tac tga ata tga gat gc–3′ | 5′–gac aat ttt cag acc tat atg g–3′ |