Percentage of donor cells after nonmyeloablative conditioning using anti-CD40L mAb (MR1) before allogeneic BMT in healthy DBA/1 mice
. | No. donor cells, mean ± SEM % . | . | |
|---|---|---|---|
| Time after allogeneic BMT, d . | TBI alone . | TBI/anti-CD40L . | |
| 42 | 27.8 ± 10.6 | 83.0 ± 1.3 | |
| 50 | 29.2 ± 13.4 | 84.8 ± 6.6 | |
| 57 | 29.2 ± 8.0 | 90.0 ± 3.9 | |
| 64 | 20.1 ± 19.8 | 83.7 ± 4.9 | |
| 77 | 3.4 ± 1.5 | 78.3 ± 11.0 | |
| 106 | 0.4 ± 0.1 | 93.9 ± 1.7 | |
| 169* | NA | 97.0 | |
| 279* | NA | 93.3 | |
| 295* | NA | 97.0 | |
. | No. donor cells, mean ± SEM % . | . | |
|---|---|---|---|
| Time after allogeneic BMT, d . | TBI alone . | TBI/anti-CD40L . | |
| 42 | 27.8 ± 10.6 | 83.0 ± 1.3 | |
| 50 | 29.2 ± 13.4 | 84.8 ± 6.6 | |
| 57 | 29.2 ± 8.0 | 90.0 ± 3.9 | |
| 64 | 20.1 ± 19.8 | 83.7 ± 4.9 | |
| 77 | 3.4 ± 1.5 | 78.3 ± 11.0 | |
| 106 | 0.4 ± 0.1 | 93.9 ± 1.7 | |
| 169* | NA | 97.0 | |
| 279* | NA | 93.3 | |
| 295* | NA | 97.0 | |
Percentages of total donor cells were analyzed by fluorescence-activated cell sorting (FACS) in peripheral blood (n = 2 mice per group; *n = 1). Level of allogeneic donor chimerism was significantly higher in mice treated with TBI plus anti-CD40L compared with mice treated with TBI alone (P < .0001). NA indicates not available.