Table 2.

Transduction of PBMCs by HIV- and MLV-based traps

HIV trap, LT†‡
MLV trap, RT†‡
Expression
Expression
Donor
% GFP+
MFI*
Integration, CpC
% trapping efficiency, expr/integr
% GFP+
MFI*
Integration, CpC
% Trapping efficiency, expr/integr
A         
   106 TU/mL   1.0   16.0   0.12   8.3   0.6   19.1   ND   ND  
   107 TU/mL   1.7   13.8   0.20   8.5   1.5   19.2   0.04   37.5  
B         
   106 TU/mL   2.8   12.6   0.32   8.7   0.8   14.1   0.02   37.7  
   107 TU/mL
 		 5.5
 		 11.5
 		 0.69
 		 8.0
 		 3.3
 		 15.0
 		 0.09
 		 36.6
 
HIV trap, LT†‡
MLV trap, RT†‡
Expression
Expression
Donor
% GFP+
MFI*
Integration, CpC
% trapping efficiency, expr/integr
% GFP+
MFI*
Integration, CpC
% Trapping efficiency, expr/integr
A         
   106 TU/mL   1.0   16.0   0.12   8.3   0.6   19.1   ND   ND  
   107 TU/mL   1.7   13.8   0.20   8.5   1.5   19.2   0.04   37.5  
B         
   106 TU/mL   2.8   12.6   0.32   8.7   0.8   14.1   0.02   37.7  
   107 TU/mL
 		 5.5
 		 11.5
 		 0.69
 		 8.0
 		 3.3
 		 15.0
 		 0.09
 		 36.6
 

TU indicates 293T transducing units; ND, not determined.

*

Mean fluorescence intensity is measured after puromycin selection

†

Paired Student ttest calculated on MFI of GFP-positive cells (% GFP+), with P < .05

‡

Unpaired Student ttest calculated on trapping efficiency, with P < .01

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