Binding of CSA, CSB, and CSC to deletion constructs of FCR3-DBLγ
. | Transfection efficiency, % . | . | . | Binding efficiency, CSA, %* . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Construct . | Exp 1 . | Exp 2 . | Exp 3 . | Exp 1 . | Exp 2 . | Exp 3 . | ||||
| FCR3-DBLγ(C1-C10) | 64 | 64 | 74 | 60 | 60 | 57 | ||||
| FCR3-DBLγ(C5-C10) | 63 | 60 | 65 | 62 | 62 | 64 | ||||
| FCR3-DBLγ(C5-C8) | 61 | 65 | 67 | 67 | 61 | 67 | ||||
| Untransfected | NA | NA | NA | 0 | 0 | 0 | ||||
. | Transfection efficiency, % . | . | . | Binding efficiency, CSA, %* . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Construct . | Exp 1 . | Exp 2 . | Exp 3 . | Exp 1 . | Exp 2 . | Exp 3 . | ||||
| FCR3-DBLγ(C1-C10) | 64 | 64 | 74 | 60 | 60 | 57 | ||||
| FCR3-DBLγ(C5-C10) | 63 | 60 | 65 | 62 | 62 | 64 | ||||
| FCR3-DBLγ(C5-C8) | 61 | 65 | 67 | 67 | 61 | 67 | ||||
| Untransfected | NA | NA | NA | 0 | 0 | 0 | ||||
Transfected 293T cells were incubated with biotinylated CSA, CSB, and CSC. FITC-conjugated streptavidin was used to detect binding of biotinylated CSA, CSB, and CSC to 293T cells. Between 1500 and 2000 293T cells were scored. The number of 293T cells that are fluorescent was determined. No fluorescent cells were seen in entire well where binding efficiency is reported as zero. The binding efficiency was calculated as follows: % binding efficiency = no. of fluorescent 293T cells × 100/total no. of 293T cells. None of the constructs bound CSB or CSC