4-1BB stimulation promotes elimination of donor CD4+ T cells
. | . | Donor . | . | Host . | |
|---|---|---|---|---|---|
| Group . | Total splenocytes . | CD4+ . | CD8+ . | B220+ . | |
| Day 1 | |||||
| Control Ig | 68.5 ± 13.3 | 1.3 ± 0.3 | 0.7 ± 0.2 | 43.5 ± 8.2 | |
| 3H3 | 64.8 ± 3.1 | 1.0 ± 0.3 | 0.6 ± 0.1 | 41.8 ± 4.0 | |
| Day 3 | |||||
| Control Ig | 110.0 ± 16.9 | 4.2 ± 1.9 | 2.1 ± 0.7 | 68.0 ± 10.3 | |
| 3H3 | 159.0 ± 35.8 | 2.6 ± 0.6 | 3.1 ± 1.2 | 80.3 ± 11.0 | |
| Day 5 | |||||
| Control Ig | 105.0 ± 17.3 | 2.5 ± 0.5 | 5.1 ± 0.7 | 45.3 ± 7.6 | |
| 3H3 | 117.5 ± 35.0 | 0.54 ± 0.5* | 2.9 ± 0.2 | 46.0 ± 9.5 | |
| Day 14 | |||||
| Control Ig | 90.8 ± 15.9 | 1.8 ± 1.2 | 5.6 ± 2.9 | 43.6 ± 14.9 | |
| 3H3 | 30.3 ± 13.1* | 0.1 ± 0.1* | 3.5 ± 1.3 | 15.0 ± 10.6* | |
| Day 21 | |||||
| Control Ig | 77.0 ± 12.6 | 2.2 ± 1.9 | 3.7 ± 2.8 | 35.6 ± 3.9 | |
| 3H3 | 64.0 ± 2.8 | 0.6 ± 0.0* | 4.8 ± 0.8 | 40.3 ± 1.0 | |
| Day 14 | |||||
| CD8-depleted† | |||||
| Control Ig | 290.8 ± 72.4 | 7.6 ± 0.6 | NT | 152.0 ± 36.3 | |
| 3H3 | 113.0 ± 12.8 | 0.6 ± 0.1* | NT | 52.2 ± 4.5* | |
. | . | Donor . | . | Host . | |
|---|---|---|---|---|---|
| Group . | Total splenocytes . | CD4+ . | CD8+ . | B220+ . | |
| Day 1 | |||||
| Control Ig | 68.5 ± 13.3 | 1.3 ± 0.3 | 0.7 ± 0.2 | 43.5 ± 8.2 | |
| 3H3 | 64.8 ± 3.1 | 1.0 ± 0.3 | 0.6 ± 0.1 | 41.8 ± 4.0 | |
| Day 3 | |||||
| Control Ig | 110.0 ± 16.9 | 4.2 ± 1.9 | 2.1 ± 0.7 | 68.0 ± 10.3 | |
| 3H3 | 159.0 ± 35.8 | 2.6 ± 0.6 | 3.1 ± 1.2 | 80.3 ± 11.0 | |
| Day 5 | |||||
| Control Ig | 105.0 ± 17.3 | 2.5 ± 0.5 | 5.1 ± 0.7 | 45.3 ± 7.6 | |
| 3H3 | 117.5 ± 35.0 | 0.54 ± 0.5* | 2.9 ± 0.2 | 46.0 ± 9.5 | |
| Day 14 | |||||
| Control Ig | 90.8 ± 15.9 | 1.8 ± 1.2 | 5.6 ± 2.9 | 43.6 ± 14.9 | |
| 3H3 | 30.3 ± 13.1* | 0.1 ± 0.1* | 3.5 ± 1.3 | 15.0 ± 10.6* | |
| Day 21 | |||||
| Control Ig | 77.0 ± 12.6 | 2.2 ± 1.9 | 3.7 ± 2.8 | 35.6 ± 3.9 | |
| 3H3 | 64.0 ± 2.8 | 0.6 ± 0.0* | 4.8 ± 0.8 | 40.3 ± 1.0 | |
| Day 14 | |||||
| CD8-depleted† | |||||
| Control Ig | 290.8 ± 72.4 | 7.6 ± 0.6 | NT | 152.0 ± 36.3 | |
| 3H3 | 113.0 ± 12.8 | 0.6 ± 0.1* | NT | 52.2 ± 4.5* | |
cGVHD was induced as described in “Materials and methods” and mice received control Ig or 3H3 on day 0. Splenocytes were analyzed by flow cytometry at the indicated days after parental cell transfer.
Values for lymphocyte subsets are shown as (mean ± SD) × 10-6. n = 4 to 6 mice per group.
ND indicates not detected.
Values of P < .05 versus the control Ig-treated cGVHD group
Donor CD8+ T cells were depleted before transfer