Composition of the populations in 19-day-old cultures initiated with EBV-infected CBMCs
Cells . | CBMC + EBV, % . | CBMC + EBV + Trx80, % . | CBMC + EBV + Trx80 + PSK, % . |
|---|---|---|---|
| CD19 B | 93.1 | 18.9 | 5.6 |
| CD4 T | 4.9 | 63.1 | 54.3 |
| CD8 T | 0.7 | 6.2 | 7.8 |
| CD56 NK | 1.9 | 6.9 | 18.6 |
| CD3 T | 5.3 | 70.7 | 62.6 |
Cells . | CBMC + EBV, % . | CBMC + EBV + Trx80, % . | CBMC + EBV + Trx80 + PSK, % . |
|---|---|---|---|
| CD19 B | 93.1 | 18.9 | 5.6 |
| CD4 T | 4.9 | 63.1 | 54.3 |
| CD8 T | 0.7 | 6.2 | 7.8 |
| CD56 NK | 1.9 | 6.9 | 18.6 |
| CD3 T | 5.3 | 70.7 | 62.6 |
The cultures were restimulated twice, on the 7th and 14th days with irradiated (50 Gy) autologous EBV-infected B cells. Half of the culture medium was replaced with fresh medium containing 100 nM Trx80 or 100 nM Trx80 and 25 μg/mL PSK. From the ninth day, 20 U/mL IL-2 was added every third day.