Numbers of cells transplanted per mouse and engraftment
Experiment no. and construct . | No. of mice . | Transduced EGFP+ cells/untransduced EGFP- cells (% EGFP+ chimerism) . | Mean PB engraftment ± SD, %* . | Mean BM engraftment ± SD, %* . |
|---|---|---|---|---|
| 1 | ||||
| Vector | 3 | 4 × 105/16 × 105 (20) | 18 ± 5 | 17 ± 3 |
| RhoH | 4 | 4 × 105/16 × 105 (20) | 10 ± 3 | 9 ± 2 |
| 2 | ||||
| Vector | 6 | 8 × 105/12 × 105 (40) | 39 ± 7 | 42 ± 9 |
| RhoH | 5 | 8 × 105/12 × 105 (40) | 18 ± 4 | 23 ± 7 |
| 3 | ||||
| Vector | 6 | 4 × 105/16 × 105 (20) | 20 ± 7 | 22 ± 4 |
| RhoH | 5 | 4 × 105/16 × 105 (20) | 4 ± 2 | 5 ± 3 |
| Vector | 6 | 8 × 105/12 × 105 (40) | 37 ± 8 | 34 ± 8 |
| RhoH | 5 | 8 × 105/12 × 105 (40) | 17 ± 5 | 12 ± 4 |
Experiment no. and construct . | No. of mice . | Transduced EGFP+ cells/untransduced EGFP- cells (% EGFP+ chimerism) . | Mean PB engraftment ± SD, %* . | Mean BM engraftment ± SD, %* . |
|---|---|---|---|---|
| 1 | ||||
| Vector | 3 | 4 × 105/16 × 105 (20) | 18 ± 5 | 17 ± 3 |
| RhoH | 4 | 4 × 105/16 × 105 (20) | 10 ± 3 | 9 ± 2 |
| 2 | ||||
| Vector | 6 | 8 × 105/12 × 105 (40) | 39 ± 7 | 42 ± 9 |
| RhoH | 5 | 8 × 105/12 × 105 (40) | 18 ± 4 | 23 ± 7 |
| 3 | ||||
| Vector | 6 | 4 × 105/16 × 105 (20) | 20 ± 7 | 22 ± 4 |
| RhoH | 5 | 4 × 105/16 × 105 (20) | 4 ± 2 | 5 ± 3 |
| Vector | 6 | 8 × 105/12 × 105 (40) | 37 ± 8 | 34 ± 8 |
| RhoH | 5 | 8 × 105/12 × 105 (40) | 17 ± 5 | 12 ± 4 |
Engraftment was determined by the mean percentage of EGFP+ cells ± SD in the PB and BM of the lethally irradiated recipient mice analyzed by flow cytometry at 6 months after transplantation in all 3 experiments.