Retroviral transduction efficiency and CFCs transplanted per mouse
. | Transduction efficiency, % ± SD . | CFCs transplanted, × 103 . | . | |
|---|---|---|---|---|
| Vector . | . | Total . | Transduced . | |
| MIG | 35 ± 14 | 8.1 | 2.8 | |
| HOXB6 | 32 ± 21 | 7.8 | 2.5 | |
| HOXB6WG | 55 ± 15 | 12 | 6.6 | |
| HOXB6NA | 42 ± 34 | 13 | 5.5 | |
. | Transduction efficiency, % ± SD . | CFCs transplanted, × 103 . | . | |
|---|---|---|---|---|
| Vector . | . | Total . | Transduced . | |
| MIG | 35 ± 14 | 8.1 | 2.8 | |
| HOXB6 | 32 ± 21 | 7.8 | 2.5 | |
| HOXB6WG | 55 ± 15 | 12 | 6.6 | |
| HOXB6NA | 42 ± 34 | 13 | 5.5 | |
For each vector, n = 15.
Transduction efficiency was defined as the percent GFP+ bone marrow cells approximately 24 hours following retroviral transduction. Transduced CFC dose was calculated as CFC transplanted × transduction efficiency.
Transduction efficiency calculated in this manner agreed with direct examination of CFC assays by fluorescent microscopy (data not shown). Results for each vector reflect 3 independent experiments (5 mice per experiment).