Panel of MoAbs used for MACS depletion of nongranulocytic cells in BM and PB populations and for subsequent flow cytometry analysis of residual contaminating cells
. | . | . | . | . | MACS depletion cocktail . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Surface molecule . | Cell type . | FACS MoAb clone . | Fluorochrome-conjugated to MoAb . | MACS MoAb clone . | PM . | MY . | bm-PMN . | pb-PMN . | |||
| CD2 | T cells, NK cells | Not used | RPA-2.10 | X | X | X | X | ||||
| CD3 | T cells | SK7 | PE | UCHT1 | X | X | — | — | |||
| CD5 | T cells | DK23 | FITC | Not used | X | X | — | — | |||
| CD10 | B-, T-cell precursors, mature neutrophils | Not used | SS2/36 | X | X | — | — | ||||
| CD11b | NK cells, monocytes, neutrophils, myeloid precursors | ICRF44 | PE, APC | ICRF44 | X | — | — | — | |||
| CD14 | Myelomonocytic cells | TÛK4 | PE, FITC | M5E2 | X | X | X | X | |||
| CD15 | Neutrophils, monocytes, eosinophils | MMA | FITC, APC | HI98 | X | X | — | — | |||
| CD16 | NK cells, monocytes, neutrophils, myeloid precursors | NKP15 | FITC | DJ130c | X | X | — | — | |||
| CD19 | B cells | 4G7 | FITC | HIB19 | X | X | X | X | |||
| CD49d | Eosinophils | 9F10 | PE | 9F10 | — | — | X | X | |||
| CD56 | NK cells | My31 | PE | B159 | X | X | X | X | |||
| CD61 | Platelets, megakaryocytes, monocytes | Y2/51 | FITC, PE | VI-PL2 | X | X | X | X | |||
| GLY-A | Erythroid cells, erythroid precursors | JC159 | R-PE | GA-R2 | X | X | X | X | |||
. | . | . | . | . | MACS depletion cocktail . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Surface molecule . | Cell type . | FACS MoAb clone . | Fluorochrome-conjugated to MoAb . | MACS MoAb clone . | PM . | MY . | bm-PMN . | pb-PMN . | |||
| CD2 | T cells, NK cells | Not used | RPA-2.10 | X | X | X | X | ||||
| CD3 | T cells | SK7 | PE | UCHT1 | X | X | — | — | |||
| CD5 | T cells | DK23 | FITC | Not used | X | X | — | — | |||
| CD10 | B-, T-cell precursors, mature neutrophils | Not used | SS2/36 | X | X | — | — | ||||
| CD11b | NK cells, monocytes, neutrophils, myeloid precursors | ICRF44 | PE, APC | ICRF44 | X | — | — | — | |||
| CD14 | Myelomonocytic cells | TÛK4 | PE, FITC | M5E2 | X | X | X | X | |||
| CD15 | Neutrophils, monocytes, eosinophils | MMA | FITC, APC | HI98 | X | X | — | — | |||
| CD16 | NK cells, monocytes, neutrophils, myeloid precursors | NKP15 | FITC | DJ130c | X | X | — | — | |||
| CD19 | B cells | 4G7 | FITC | HIB19 | X | X | X | X | |||
| CD49d | Eosinophils | 9F10 | PE | 9F10 | — | — | X | X | |||
| CD56 | NK cells | My31 | PE | B159 | X | X | X | X | |||
| CD61 | Platelets, megakaryocytes, monocytes | Y2/51 | FITC, PE | VI-PL2 | X | X | X | X | |||
| GLY-A | Erythroid cells, erythroid precursors | JC159 | R-PE | GA-R2 | X | X | X | X | |||
MoAb clones used for MACS depletion and fluorescence activated cell sorting (FACS) analysis were directed against different epitopes of the same surface markers with exception of the anti-CD49d and anti-CD11b MoAbs.
— indicates not used.