Nucleic acid sequences of primers and probes used in quantitative RT-PCR reactions
Accession no. and oligonucleotide . | Sequence . |
|---|---|
| U81375 | |
| hENT1-F | 5′–CACCAGCCTCAGGACAGATACAA |
| hENT1-R | 5′–GTGAAATACTGAGTGGCCGTCAT |
| hENT1-P | 5′–FAM–CCACGGGAGCAGCGTTCCCA |
| U81375 | |
| hENT2-F | 5′–ATGAGAACGGGATTCCCAGTAG |
| hENT2-R | 5′–GCTCTGATTCCGGCTCCTT |
| hENT2-P | 5′–TET–CAGAAAGTAGCTCTGACCCTGGATCTTGACCT |
| AF305210 | |
| hCNT3-F | 5′–GGGTCCCTAGGAATCGTGATC |
| hCNT3-R | 5′–CGAGGCGATATCACGCTTTC |
| hCNT3-P | 5′–FAM–CGGACTCACATCCATGGCTCCTTC |
| M60527 | |
| dCK-F | 5′–AAACCTGAACGATGGTCTTTTACC |
| dCK-R | 5′–CTTTGAGCTTGCCATTCAGAGA |
| dCK-P | 5′–FAM–CAAACATATGCCTGTCTCAGTCGAATAAGAGCTC |
| NM_002526 | |
| CD73-F | 5′–TCCGGTCGCCCATTGA |
| CD73-R | 5′–GCCTCCAAAGGGCAATACAG |
| CD73-P | 5′–FAM–ACGCAACAATGGCACAATTACCTGGG |
| D38524 | |
| CN-II-F | 5′–ACCTGCTGTATTACCCTTTCAGCTA |
| CN-II-R | 5′–GCTCCACCGTTGATTCATGA |
| CN-II-P | 5′–FAM–CTCTTCAGGGCTGCCCATGTCTTGA |
| NM_014595 | |
| dNT1-F | 5′–GGACACGCAGGTCTTCATCTG |
| dNT1-R | 5′–GCGGTACTTCTCACCCACACA |
| dNT1-P | 5′–FAM–CCAGCCCCCTGCTGAAGTACCACC |
Accession no. and oligonucleotide . | Sequence . |
|---|---|
| U81375 | |
| hENT1-F | 5′–CACCAGCCTCAGGACAGATACAA |
| hENT1-R | 5′–GTGAAATACTGAGTGGCCGTCAT |
| hENT1-P | 5′–FAM–CCACGGGAGCAGCGTTCCCA |
| U81375 | |
| hENT2-F | 5′–ATGAGAACGGGATTCCCAGTAG |
| hENT2-R | 5′–GCTCTGATTCCGGCTCCTT |
| hENT2-P | 5′–TET–CAGAAAGTAGCTCTGACCCTGGATCTTGACCT |
| AF305210 | |
| hCNT3-F | 5′–GGGTCCCTAGGAATCGTGATC |
| hCNT3-R | 5′–CGAGGCGATATCACGCTTTC |
| hCNT3-P | 5′–FAM–CGGACTCACATCCATGGCTCCTTC |
| M60527 | |
| dCK-F | 5′–AAACCTGAACGATGGTCTTTTACC |
| dCK-R | 5′–CTTTGAGCTTGCCATTCAGAGA |
| dCK-P | 5′–FAM–CAAACATATGCCTGTCTCAGTCGAATAAGAGCTC |
| NM_002526 | |
| CD73-F | 5′–TCCGGTCGCCCATTGA |
| CD73-R | 5′–GCCTCCAAAGGGCAATACAG |
| CD73-P | 5′–FAM–ACGCAACAATGGCACAATTACCTGGG |
| D38524 | |
| CN-II-F | 5′–ACCTGCTGTATTACCCTTTCAGCTA |
| CN-II-R | 5′–GCTCCACCGTTGATTCATGA |
| CN-II-P | 5′–FAM–CTCTTCAGGGCTGCCCATGTCTTGA |
| NM_014595 | |
| dNT1-F | 5′–GGACACGCAGGTCTTCATCTG |
| dNT1-R | 5′–GCGGTACTTCTCACCCACACA |
| dNT1-P | 5′–FAM–CCAGCCCCCTGCTGAAGTACCACC |
F indicates forward (5′) primer; R, reverse (3′) primer; P, probe; FAM, 6-carboxy-fluorescein; and TET, tetrochloro-6-carboxy-fluorescein.