Table 2. Combination of anti-CD28 mAb... | American Society of Hematology

Table 2.

Combination of anti-CD28 mAb and rapamycin leads to long-term donor T- and B-cell reconstitution

		Donor T cells					Donor B cells
	Survival, %	%	×10⁶	cpm × 1000	%	×10⁶	cpm × 1000
BM alone	100	18 ± 3	22 ± 5	11 ± 2	60 ± 4	73 ± 10	53 ± 11
Control	0	—	—	—	—	—	—
Rapamycin	0	—	—	—	—	—	—
αCD28	40	25 ± 3^*	4 ± 1†	5 ± 2†	25 ± 10†	4 ± 2†	34 ± 7†
αCD28 + rapamycin	57	23 ± 2^*	13 ± 7†	9 ± 2^*	62 ± 7^*	35 ± 5†	56 ± 13^*

		Donor T cells					Donor B cells
	Survival, %	%	×10⁶	cpm × 1000	%	×10⁶	cpm × 1000
BM alone	100	18 ± 3	22 ± 5	11 ± 2	60 ± 4	73 ± 10	53 ± 11
Control	0	—	—	—	—	—	—
Rapamycin	0	—	—	—	—	—	—
αCD28	40	25 ± 3^*	4 ± 1†	5 ± 2†	25 ± 10†	4 ± 2†	34 ± 7†
αCD28 + rapamycin	57	23 ± 2^*	13 ± 7†	9 ± 2^*	62 ± 7^*	35 ± 5†	56 ± 13^*

Lethally irradiated (1200 cGy) B10.BR recipients were given transplants of 15 × 10⁶ T-cell-depleted marrow cells plus 25 × 10⁶ splenocytes from C57BL/6 donors. Recipients were treated intraperitoneally with either anti-CD28 mAb (αCD28) at 100 μg × 1 or rapamycin (rapamycin) at 1.5 mg/kg × 14 days or both (αCD28 + rapamycin). Surviving recipients were killed on day 120 after transplantation. Splenocytes were stained for expression of CD4, CD8, B220 and H2K^b. T-cell function was measured by stimulating splenocytes with Concanavalin A for 3 days, and B cell function was measured by stimulating splenocytes with LPS for 3 days. Data are presented as average counts per minute (cpm) ± SD. Results are representative of 2 replicate experiments. — indicates that no data are available, because all mice in this group died before day 120.

P > .05 compared with BM alone

†

P < .05 compared with BM alone

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