Sequence of human gene–specific primers used for PCR
Gene . | Forward primer (5′-3′) . | Reverse primer (5′-3′) . | Amplicon, bp . |
|---|---|---|---|
| CD3δ | TTCCGGTACCTGTGAGTCAGC | GGTACAGTTGGTAATGGCTGC | 636 |
| CD3ϵ | AGTTGGCGTTTGGGGGCAAGATGGTAATGAAGAAA | CCCAGGAAACAGGGAGTCGCAGGGGGACTGGAGAG | 640 |
| CD3γ | GGGCTGCTCCACGCTTTTGC | TTTTCCCCAATAGGTGGCGC | 817 |
| CD3ζ | CTCTGCCTCCCAGCCTCTTT | GCGTCGTAGGTGTCCTTGGT | 481 |
| CD45 | TTCAACTTATACCCTTCGTGTC | CCTGCTTTACTTTGTCCACTTC | 463 |
| Flk-1 | ATGCACGGCATCTGGGAATC | GTCACTGTCCTGCAAGTTGCTGTC | 573 |
| Flt-3 | CAAGTGCTGTGCATACAATTCCC | ACCTGTACCATCTGTAGCTGG | 210 |
| GAPDH | TCCAAAATCAAGTGGGGCGAT | TTCTAGACGGCAGGTCAGGTC | 475 |
| GATA-1 | CTCCCTGTCCCCAATAGTGC | GTCCTTCGGCTGCTCCTGTG | 520 |
| GATA-2 | GCTTCCCTCTCTGAAATAGCCGAA | CAGAATCTAAGCTCGGGACACGTT | 771 |
| GATA-3 | TGCAGGAGCAGTATCATGAAGCCT | GCATCAAACAACTGTGGCCAGTGA | 406 |
| mb-1 | TCCAAGCTCTGCCTGCCACCAT | GACTGCTGGTATGACTCGTTGC | 330 |
| pre-Tα | AGTACACAGCCCATGCATCTGTCA | AATGCTCCAAGACTGGAGGAAGGA | 446 |
| SCL | ATGGTGCAGCTGAGTCCTCC | TCTCATTCTTGCTGAGCTTC | 331 |
| VpreB | TTTGTCTACTGCACAGGTTGTGG | TGCAGTGGGTTCCATTTCTTCC | 386 |
| β-Actin | TGACGGGGTCACCCACACTGTGCCCATCTA | CTAGAAGCATTGCGGTGGACGATGGAGGG | 650 |
Gene . | Forward primer (5′-3′) . | Reverse primer (5′-3′) . | Amplicon, bp . |
|---|---|---|---|
| CD3δ | TTCCGGTACCTGTGAGTCAGC | GGTACAGTTGGTAATGGCTGC | 636 |
| CD3ϵ | AGTTGGCGTTTGGGGGCAAGATGGTAATGAAGAAA | CCCAGGAAACAGGGAGTCGCAGGGGGACTGGAGAG | 640 |
| CD3γ | GGGCTGCTCCACGCTTTTGC | TTTTCCCCAATAGGTGGCGC | 817 |
| CD3ζ | CTCTGCCTCCCAGCCTCTTT | GCGTCGTAGGTGTCCTTGGT | 481 |
| CD45 | TTCAACTTATACCCTTCGTGTC | CCTGCTTTACTTTGTCCACTTC | 463 |
| Flk-1 | ATGCACGGCATCTGGGAATC | GTCACTGTCCTGCAAGTTGCTGTC | 573 |
| Flt-3 | CAAGTGCTGTGCATACAATTCCC | ACCTGTACCATCTGTAGCTGG | 210 |
| GAPDH | TCCAAAATCAAGTGGGGCGAT | TTCTAGACGGCAGGTCAGGTC | 475 |
| GATA-1 | CTCCCTGTCCCCAATAGTGC | GTCCTTCGGCTGCTCCTGTG | 520 |
| GATA-2 | GCTTCCCTCTCTGAAATAGCCGAA | CAGAATCTAAGCTCGGGACACGTT | 771 |
| GATA-3 | TGCAGGAGCAGTATCATGAAGCCT | GCATCAAACAACTGTGGCCAGTGA | 406 |
| mb-1 | TCCAAGCTCTGCCTGCCACCAT | GACTGCTGGTATGACTCGTTGC | 330 |
| pre-Tα | AGTACACAGCCCATGCATCTGTCA | AATGCTCCAAGACTGGAGGAAGGA | 446 |
| SCL | ATGGTGCAGCTGAGTCCTCC | TCTCATTCTTGCTGAGCTTC | 331 |
| VpreB | TTTGTCTACTGCACAGGTTGTGG | TGCAGTGGGTTCCATTTCTTCC | 386 |
| β-Actin | TGACGGGGTCACCCACACTGTGCCCATCTA | CTAGAAGCATTGCGGTGGACGATGGAGGG | 650 |