Flow cytometric characteristics and TCR clonality of the 5 patients with SzS studied
Patient no. . | 1 . | 2 . | 3 . | 4 . | 5 . |
|---|---|---|---|---|---|
| Tumor burden | High | Low | High | High | Medium |
| % SzS cells (absolute no.) | 82 (2330) | 5 (60) | 85 (NA) | 85 (3160) | 20 (120) |
| % CD4 cells (absolute no.) | 92 (2600) | 44 (390) | 24 (234) | 88 (3265) | NA |
| % CD8 cells (absolute no.) | 5 (151) | 23 (205) | 24 (234) | 5 (197) | NA |
| Tumor phenotype* | 4+7− | 4+/−7+ | 4+7+ | 4+7− | 4+7− |
| % CD40L+ on CD4+CD7+ | 14 | 27 | 3 | 0 | 1 |
| % CD40L+ on CD4+ | 3.4 | 25 | 3 | 0 | 1 |
| % CD40L on tumor cells† | 0 | 0 | 3 | 0 | 0 |
| TCRγ clonality assay | Positive | Positive | Positive | Positive | Positive |
Patient no. . | 1 . | 2 . | 3 . | 4 . | 5 . |
|---|---|---|---|---|---|
| Tumor burden | High | Low | High | High | Medium |
| % SzS cells (absolute no.) | 82 (2330) | 5 (60) | 85 (NA) | 85 (3160) | 20 (120) |
| % CD4 cells (absolute no.) | 92 (2600) | 44 (390) | 24 (234) | 88 (3265) | NA |
| % CD8 cells (absolute no.) | 5 (151) | 23 (205) | 24 (234) | 5 (197) | NA |
| Tumor phenotype* | 4+7− | 4+/−7+ | 4+7+ | 4+7− | 4+7− |
| % CD40L+ on CD4+CD7+ | 14 | 27 | 3 | 0 | 1 |
| % CD40L+ on CD4+ | 3.4 | 25 | 3 | 0 | 1 |
| % CD40L on tumor cells† | 0 | 0 | 3 | 0 | 0 |
| TCRγ clonality assay | Positive | Positive | Positive | Positive | Positive |
NA indicates not available.
PBMCs from patients with SzS were double-stained for CD4 and CD7 expression. Cell populations showing an overrepresentation compared with healthy donors were assigned as tumor cells. Representative dot plots are shown in Figure 2.
Total PBMCs were stimulated with anti-CD3, and CD40L expression was assessed as in Figure 2.