Sequence of the primers for PCR amplification of the chicken ACE, AT receptor, renin, AGT, and GAPDH cDNAs
Primers . | Sense and antisense primer sequences . | PCR product, length . |
|---|---|---|
| cACE | 5′ CGC TGG GGC ACT CCT GGA GTA TT 3′ | 630 bp |
| 5′ GGA CCA TGA CTG GGC CCA CAT 3 | ||
| cAT | 5′ CTGTTTCAGGAAGGCACAGTTAC 3′ | 519 bp |
| 5′ TGTCATATCGAAAGCCACAGAC 3′ | ||
| cRenin | 5′ CCCTGATACGAGCACAGGAG 3′ | 495 bp |
| 5′ GCACTTGCTTGATCTCAGCCCC 3′ | ||
| cAGT | 5′ CATGACTGACACCAGTGAGACC 3′ | 546 bp |
| 5′ GGTGATGGGAAATGTGCATCC 3′ | ||
| cGAPDH | 5′ TGT GAT CAA TGG GCA CGC CAT CACTAT 3′ | 822 bp |
| 5′ GCT GTG TGC TTG GCT CAC TCC TTG G 3′ |
Primers . | Sense and antisense primer sequences . | PCR product, length . |
|---|---|---|
| cACE | 5′ CGC TGG GGC ACT CCT GGA GTA TT 3′ | 630 bp |
| 5′ GGA CCA TGA CTG GGC CCA CAT 3 | ||
| cAT | 5′ CTGTTTCAGGAAGGCACAGTTAC 3′ | 519 bp |
| 5′ TGTCATATCGAAAGCCACAGAC 3′ | ||
| cRenin | 5′ CCCTGATACGAGCACAGGAG 3′ | 495 bp |
| 5′ GCACTTGCTTGATCTCAGCCCC 3′ | ||
| cAGT | 5′ CATGACTGACACCAGTGAGACC 3′ | 546 bp |
| 5′ GGTGATGGGAAATGTGCATCC 3′ | ||
| cGAPDH | 5′ TGT GAT CAA TGG GCA CGC CAT CACTAT 3′ | 822 bp |
| 5′ GCT GTG TGC TTG GCT CAC TCC TTG G 3′ |
GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; bp, base pair.