Time-dependent changes in MCV, SD of MCV, and MCHC of normal RBCs during Ca2+-induced dehydration, in the presence and absence of 1 mM sodium orthovanadate in the RBC suspension
. | Control . | . | . | Vanadate . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Time, min . | MCV, fL . | SD of MCV . | MCHC, g/dL . | MCV, fL . | SD of MCV . | MCHC, g/dL . | ||||
| 0 | 81 | 11 | 33.5 | 79 | 11 | 34.9 | ||||
| 0.25 | 77 | 10 | 34.9 | 76 | 10 | 36.2 | ||||
| 0.75 | 75 | 10 | 36.1 | 73.1 | 9.9 | 36.8 | ||||
| 1.27 | 72.5 | 9.7 | 36.9 | 70.9 | 9.7 | 37.7 | ||||
| 1.77 | 70.6 | 9.6 | 38.7 | 69.1 | 9.4 | 38.6 | ||||
| 2.27 | 69.1 | 9.4 | 38.6 | 67.5 | 9.3 | 39.5 | ||||
| 2.75 | 68.0 | 9.0 | 39.2 | 66.4 | 9.0 | 40.9 | ||||
| 3.25 | 67.2 | 8.7 | 40.5 | 65.7 | 8.8 | 40.8 | ||||
| 3.77 | 66.9 | 8.5 | 40.0 | 65.3 | 8.6 | 41.9 | ||||
| 4.27 | 66.8 | 8.2 | 40.3 | 65.3 | 8.5 | 41.8 | ||||
| 4.77 | 66.9 | 8.0 | 40.3 | 65.6 | 8.4 | 41.0 | ||||
. | Control . | . | . | Vanadate . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Time, min . | MCV, fL . | SD of MCV . | MCHC, g/dL . | MCV, fL . | SD of MCV . | MCHC, g/dL . | ||||
| 0 | 81 | 11 | 33.5 | 79 | 11 | 34.9 | ||||
| 0.25 | 77 | 10 | 34.9 | 76 | 10 | 36.2 | ||||
| 0.75 | 75 | 10 | 36.1 | 73.1 | 9.9 | 36.8 | ||||
| 1.27 | 72.5 | 9.7 | 36.9 | 70.9 | 9.7 | 37.7 | ||||
| 1.77 | 70.6 | 9.6 | 38.7 | 69.1 | 9.4 | 38.6 | ||||
| 2.27 | 69.1 | 9.4 | 38.6 | 67.5 | 9.3 | 39.5 | ||||
| 2.75 | 68.0 | 9.0 | 39.2 | 66.4 | 9.0 | 40.9 | ||||
| 3.25 | 67.2 | 8.7 | 40.5 | 65.7 | 8.8 | 40.8 | ||||
| 3.77 | 66.9 | 8.5 | 40.0 | 65.3 | 8.6 | 41.9 | ||||
| 4.27 | 66.8 | 8.2 | 40.3 | 65.3 | 8.5 | 41.8 | ||||
| 4.77 | 66.9 | 8.0 | 40.3 | 65.6 | 8.4 | 41.0 | ||||
Results shown are from 1 experiment, representative of 5 similar experiments on RBCs from 4 donors. All measurements were performed by flow cytometry on the Advia 120 Hematology System (see “Materials and methods”). MCVs correspond to the Advia-measured volume distributions shown in Figure 2 (top panels). MCV, SD of MCV, and MCHC values are those plotted as a function of time in Figure 2 (lower panels). Times shown are the intervals between addition of ionophore A23187 to the RBC suspension (t = 0) and the delivery of the sample into the Advia. RBCs were suspended in solution 15K (see “Solutions”).